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Comparative transcriptomic and metabolic analysis of wild and domesticated wheat genotypes reveals differences in chemical and physical defense responses against aphids Zhaniya S Batyrshina BenGurion
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How to fill out comparative transcriptome and metabolic

01
Identify the species or conditions to compare in your transcriptome and metabolic analysis.
02
Collect samples from each condition or species for RNA extraction and metabolite analysis.
03
Perform RNA sequencing (RNA-seq) on the samples to obtain transcriptomic data.
04
Process the RNA-seq data using bioinformatics tools to align reads and quantify gene expression levels.
05
Extract metabolic profiles using mass spectrometry or chromatography techniques.
06
Normalize and analyze the transcriptomic and metabolomic data to identify significant differences.
07
Use statistical and bioinformatics tools to correlate gene expression with metabolic changes.
08
Interpret the results, identifying pathways and functional groups affected by the variations.

Who needs comparative transcriptome and metabolic?

01
Researchers studying genomic and metabolic interactions in various organisms.
02
Biologists interested in the differences between species or conditions at the molecular level.
03
Pharmaceutical companies developing new drugs based on metabolic profiles.
04
Agricultural scientists aiming to improve crop traits through understanding metabolic pathways.
05
Clinicians looking to correlate gene expression with metabolic disorders.
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Comparative transcriptome and metabolic refers to the analysis and comparison of gene expression patterns (transcriptomes) and metabolic pathways between different organisms or conditions. This approach helps elucidate functional differences and similarities at the molecular level.
Researchers and institutions involved in biological studies, especially those focusing on genetics, genomics, and metabolism, are required to file comparative transcriptome and metabolic analyses. This includes academic institutions, biotech companies, and pharmaceutical companies conducting relevant research.
Filling out comparative transcriptome and metabolic analysis typically involves collecting high-throughput sequencing data, aligning transcripts, quantifying gene expression levels, analyzing metabolic pathways, and comparing these across the studied conditions or organismal systems using relevant bioinformatics tools.
The purpose of comparative transcriptome and metabolic analyses is to identify variations in gene expression and metabolic processes between different organisms or conditions. This can provide insights into evolution, adaptation, disease mechanisms, and potential therapeutic targets.
Information that must be reported includes the methodology used for transcriptome and metabolic analysis, the data obtained (such as gene expression levels and metabolic profiles), statistical analyses performed, and any significant findings related to the comparisons made.
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