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Annexure 1Ziehlneelsen staining procedure 1. A new unscratched slide is selected and the slide is labelled with the Laboratory Serial Number with a diamond marking pencil. 2. A smear is made from
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01
Prepare the slide with a smear of the sample.
02
Fix the smear by passing it through a flame (if using a glass slide).
03
Flood the slide with the primary stain, carbol fuchsin, and heat gently for 5 minutes.
04
Allow the slide to cool and then wash it with water.
05
Decolorize the smear with acid-alcohol solution for approximately 2-3 minutes until no more color runs off.
06
Wash the slide again with water.
07
Counterstain the slide with methylene blue for about 1 minute.
08
Wash the slide again with water and gently dry it.
09
Examine the slide under a microscope using oil immersion at 1000x magnification.

Who needs ziehl-neelsen staining procedure?

01
Microbiologists needing to identify mycobacteria.
02
Clinicians diagnosing tuberculosis or other related infections.
03
Laboratories conducting research on mycobacterial diseases.
04
Public health officials monitoring public health threats related to mycobacteria.
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The Ziehl-Neelsen staining procedure is a method used to identify acid-fast bacilli, particularly Mycobacterium tuberculosis, in clinical samples. It involves applying a special stain (carbol fuchsin) to the sample, followed by decolorization with acid-alcohol and counterstaining with methylene blue.
Healthcare professionals, such as microbiologists, pathologists, and laboratory technicians, are required to perform and file the Ziehl-Neelsen staining procedure when diagnosing tuberculosis or other mycobacterial infections.
To fill out the Ziehl-Neelsen staining procedure, document the patient’s details, sample information, staining results (positive or negative for acid-fast bacilli), and any relevant observations noted during the procedure in the laboratory report.
The purpose of the Ziehl-Neelsen staining procedure is to detect the presence of acid-fast bacteria, particularly for diagnosing infections caused by Mycobacterium species, including tuberculosis.
The information that must be reported includes patient identification, date of the test, type of specimen, results of the staining (positive or negative), and any relevant notes on the presence of acid-fast bacilli.
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