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El kit de transfección TransIT-PRO® ha sido específicamente desarrollado para la producción de proteínas en células CHO y 293 en suspensión. Incluye el reactivo de transfección TransIT-PRO® y el reactivo PRO Boost, que puede mejorar la expresión de proteínas. Es compatible con medios químicos definidos y no requiere un cambio de medio después de la transfección.
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How to fill out transit-pro transfection kit protocol
How to fill out transit-pro transfection kit protocol
01
Gather all necessary materials: transit-pro transfection kit, plasmid DNA, and appropriate culture medium.
02
Prepare cells by seeding them in a suitable plate or dish a day before transfection to ensure they are 70-90% confluent.
03
Dilute the appropriate amount of plasmid DNA in a buffer (usually Opti-MEM or similar).
04
In a separate tube, mix the transit-pro reagent with the diluted DNA according to the kit's instructions.
05
Incubate the mixture for the recommended time (usually around 20-30 minutes) to allow complex formation.
06
Add the DNA-reagent complexes to the prepared cells gently to ensure even distribution.
07
Incubate the cells under optimal conditions for transfection, such as 37°C in a CO2 incubator.
08
After the incubation period (usually 24-48 hours), assess transfection efficiency using appropriate methods, such as fluorescence microscopy or flow cytometry.
Who needs transit-pro transfection kit protocol?
01
Researchers and scientists working in molecular biology and genetic research.
02
Laboratories focusing on gene expression studies, CRISPR experiments, or RNA interference.
03
Biotechnology companies developing therapeutic agents or gene therapies.
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What is transit-pro transfection kit protocol?
The transit-pro transfection kit protocol involves a series of steps for introducing nucleic acids into cells using the transit-pro reagent, optimizing conditions for successful transfection.
Who is required to file transit-pro transfection kit protocol?
Researchers and laboratory personnel using the transit-pro transfection kit in their experiments are required to file the protocol, particularly when reporting results.
How to fill out transit-pro transfection kit protocol?
To fill out the transit-pro transfection kit protocol, one must provide details such as the cell type used, the amount of nucleic acid, reagent volumes, incubation times, and any modifications made to the standard protocol.
What is the purpose of transit-pro transfection kit protocol?
The purpose of the transit-pro transfection kit protocol is to standardize the process of introducing genetic material into cells for research purposes, ensuring reproducibility and reliability of results.
What information must be reported on transit-pro transfection kit protocol?
Information that must be reported includes the type of cells transfected, the nucleic acid used, concentrations, the quantities of the transit-pro reagent, incubation conditions, and the outcomes observed.
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