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Comparative transcriptome and metabolic analysis of wild and domesticated wheat genotypes reveals differences in chemical and physical defense responses against aphids Zhaniya S Batyrshina BenGurion University of the Negev Beery Yaakov BenGurion University of the Negev Reut Shavit BenGurion University of the Negev Anuradha Singh BenGurion University of the Negev Vered Tzin ( vtzin@bgu.ac.il ) https://orcid.org/000000025912779XResearch article Keywords: Aphid infestation; benzoxazinoids;

How to fill out comparative transcriptome and metabolic

How to fill out comparative transcriptome and metabolic

1. Gather biological samples from the organisms or conditions you wish to compare.
2. Extract RNA from each sample using a consistent method to ensure quality.
3. Prepare cDNA libraries from the extracted RNA for sequencing.
4. Sequence the cDNA libraries using high-throughput sequencing technologies.
5. Perform quality control on the sequencing data to remove low-quality reads.
6. Align the cleaned reads to a reference genome or transcriptome.
7. Quantify gene expression levels across samples using appropriate software tools.
8. Conduct metabolic profiling using techniques like GC-MS or LC-MS to analyze metabolites.
9. Compare gene expression and metabolic profiles to identify significant differences.
10. Interpret the results in the context of biological relevance and research objectives.

Who needs comparative transcriptome and metabolic?

1. Researchers studying gene expression differences in various biological conditions.
2. Biologists conducting comparative studies across species or strains.
3. Pharmaceutical companies looking to understand metabolic pathways for drug development.
4. Agricultural scientists enhancing crop traits through genetic and metabolic analyses.
5. Environmental scientists assessing how species respond to environmental changes.

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What is comparative transcriptome and metabolic?

Comparative transcriptome and metabolic analysis involves comparing the gene expression and metabolic profiles of different organisms or conditions to understand functional differences and evolutionary relationships.

Who is required to file comparative transcriptome and metabolic?

Researchers and institutions conducting studies that involve examining the differences in transcriptome and metabolism between organisms or conditions are typically required to file comparative transcriptome and metabolic reports.

How to fill out comparative transcriptome and metabolic?

To fill out a comparative transcriptome and metabolic report, one must collect data on gene expression and metabolic profiles, analyze the results using appropriate statistical methods, and compile the findings into a structured format as required by the reporting guidelines.

What is the purpose of comparative transcriptome and metabolic?

The purpose of comparative transcriptome and metabolic studies is to gain insights into the biological differences between organisms, understand disease mechanisms, and identify potential biomarkers or therapeutic targets.

What information must be reported on comparative transcriptome and metabolic?

The report should include details on sample descriptions, experimental conditions, methods used for transcriptome and metabolic analysis, key findings, and any relevant statistical analyses to support the conclusions.