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This manual provides comprehensive instructions for conducting the Serine Threonine Kinase assay using the PamStation®12 instrument, outlining its intended use, assay protocols, sample preparation, and troubleshooting tips.
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How to fill out serine threonine kinase assay

01
Prepare the assay buffer and reagents according to the protocol.
02
Dilute the serine threonine kinase sample in the assay buffer.
03
Add the substrate specific for the serine threonine kinase to the sample.
04
Incubate the mixture at the specified temperature and for the specified time.
05
Stop the reaction by adding a stopping solution.
06
Measure the absorbance or fluorescence using a suitable plate reader.
07
Analyze the results by comparing against a standard curve or control samples.

Who needs serine threonine kinase assay?

01
Researchers studying cellular signaling pathways.
02
Biochemists working on protein interactions and modifications.
03
Pharmaceutical companies developing kinase inhibitors.
04
Clinical laboratories performing diagnostics related to cancer or other diseases.
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The serine threonine kinase assay is a laboratory test used to measure the activity of serine threonine kinases, which are enzymes that are involved in various cellular processes including cell signaling, metabolism, and cell division.
Researchers and laboratories conducting studies that involve the analysis of serine threonine kinases, particularly in the fields of biochemistry, pharmacology, and molecular biology, are typically required to perform these assays.
Filling out a serine threonine kinase assay typically involves documenting the assay conditions such as substrate concentration, enzyme concentrations, incubation times, and the results of the activity measurement. This is often done in a standard lab notebook or assay report format.
The purpose of the serine threonine kinase assay is to evaluate the functional activity of serine threonine kinases, to help understand their role in cellular signaling pathways, and to test the effects of potential inhibitors or activators on these enzymes.
The information that must be reported on a serine threonine kinase assay includes the assay conditions, enzyme sources, substrate details, measurement methods, and results, including activity levels and any controls used in the experiment.
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Relevant keywords

Serine Threonine Kinase PamStation®12 Assay Protocol Sample Preparation Kinase Activity Profiling PamChip® arrays Recombinant Kinases Inhibitors Research Purposes Fluorescent Detection

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