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Engineering development and disease in organoidsAugust 1921, 2024 San Diego, CA, USASupporting JournalsOrganoids are 3D cell culture systems derived from stem cells that recapitulate the cell composition
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01
Gather necessary materials: organoid culture media, patient-derived cells, and appropriate 3D culture plates.
02
Isolate the cells from the tissue sample by using enzymatic digestion or mechanical dissociation.
03
Count the cells using a hemocytometer and adjust the concentration to the desired level for the organoid culture.
04
Mix the isolated cells with the organoid culture media in a sterile environment.
05
Plate the cell mixture into the 3D culture plates or other appropriate scaffolds.
06
Incubate the plates under controlled environmental conditions: temperature, humidity, and carbon dioxide levels.
07
Monitor the organoids periodically, changing the culture media as needed.
08
Perform assays or analyses on the organoids once they have grown to the desired size.

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Researchers in cancer biology studying tumor growth and drug responses.
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Organoids are three-dimensional cell cultures that are derived from stem cells and can mimic the structure and function of actual organs in the body.
Researchers and institutions working with organoids, especially in a regulatory context or for clinical applications, are typically required to file relevant information.
To file information about organoids, one would typically need to collect data on the type of organoid, its origin, the methods used for its creation, and any relevant ethical approvals.
The purpose of organoids is to provide a more accurate model for studying human biology, disease mechanisms, and drug responses, thereby enhancing research and therapeutic developments.
Information that must be reported includes the specific type of organoid, its source, the methodology used for its development, any genetic modifications, and data on experiments conducted with it.
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