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RECOMBINANT PHAGE ENDOLYSIN AS ANTIBIOFILM AGENT AGAINST STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSAYEONG ZI HUIBACHELOR OF SCIENCE (HONS) BIOMEDICAL SCIENCEFACULTY OF SCIENCE UNIVERSITI TUNKU
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How to fill out recombinant phage endolysin

How to fill out recombinant phage endolysin
01
Prepare your bacterial strain and plasmid expressing the recombinant phage endolysin.
02
Culture the bacteria in a suitable growth medium until they reach the desired optical density.
03
Induce the expression of the recombinant phage endolysin by adding the appropriate inducer (if necessary).
04
Harvest the bacteria by centrifugation and resuspend in lysis buffer.
05
Lyse the bacterial cells using a suitable method (e.g., sonication or freeze-thaw cycles).
06
Clarify the lysate by centrifugation to remove cell debris.
07
Purify the recombinant endolysin using affinity chromatography or other purification techniques.
08
Analyze the purity and activity of the endolysin using SDS-PAGE and other methods.
09
Store the purified recombinant phage endolysin under appropriate conditions for future use.
Who needs recombinant phage endolysin?
01
Researchers studying bacterial infections.
02
Pharmaceutical companies developing antibacterial therapies.
03
Laboratories involved in phage therapy and antimicrobial resistance research.
04
Food industry professionals ensuring food safety from pathogenic bacteria.
05
Veterinarians treating bacterial infections in animals.
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What is recombinant phage endolysin?
Recombinant phage endolysin is an enzyme derived from bacteriophages that can break down bacterial cell walls, leading to the lysis of the bacteria. It is genetically engineered to enhance its efficacy against specific bacterial strains.
Who is required to file recombinant phage endolysin?
Researchers and companies developing recombinant phage endolysins for therapeutic use or commercialization are required to file relevant documentation with regulatory authorities.
How to fill out recombinant phage endolysin?
To fill out recombinant phage endolysin documentation, one must provide relevant details concerning its composition, the method of production, intended use, and results from preliminary tests demonstrating its safety and effectiveness.
What is the purpose of recombinant phage endolysin?
The purpose of recombinant phage endolysin is to provide an alternative treatment for bacterial infections, particularly in the face of rising antibiotic resistance, by specifically targeting and destroying bacterial cells.
What information must be reported on recombinant phage endolysin?
Information that must be reported includes the molecular details of the endolysin, production methods, validation of specificity and efficacy against target bacteria, safety assessments, and proposed applications.
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