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bioRxiv preprint doi: https://doi.org/10.1101/2021.09.23.461506; this version posted September 23, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder.
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01
Collect sample material (e.g., tissue or cell samples).
02
Extract RNA from the samples using an appropriate extraction method.
03
Quantify and assess the quality of the extracted RNA.
04
Prepare libraries for sequencing by converting RNA into complementary DNA (cDNA).
05
Perform sequencing using a suitable platform (e.g., Illumina, PacBio).
06
Process the raw sequencing data using bioinformatics tools to align reads to a reference genome.
07
Analyze the transcriptomic and miRNA data using specialized software to identify differentially expressed genes and miRNA profiles.

Who needs transcriptome and mirnaome reveal?

01
Researchers in molecular biology and genetics.
02
Oncologists studying cancer-related gene expression.
03
Biotechnologists developing RNA-based therapeutics.
04
Academics conducting functional genomics investigations.
05
Pharmacologists aiming to understand drug responses at the RNA level.
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The transcriptome reveals the complete set of RNA transcripts produced by the genome under specific circumstances, while the miRNAome refers to the complete set of microRNAs expressed in a cell or organism, both of which provide insights into gene expression and regulation.
Researchers and institutions involved in genomic studies, as well as those submitting data related to RNA sequencing or molecular studies for regulatory purposes, are required to file transcriptome and miRNAome reveals.
Filling out a transcriptome and miRNAome reveal typically involves providing detailed information on sample preparation, sequencing methods, data analysis processes, and specific findings related to gene expression and microRNA profiles.
The purpose of transcriptome and miRNAome reveals is to communicate findings related to RNA expression levels, regulatory mechanisms, and to facilitate the understanding of cellular processes and disease mechanisms through the lens of gene expression.
Required information usually includes details on the experimental design, RNA extraction methods, sequencing platforms used, quality control checks, data processing protocols, and significant outcomes related to the identified transcripts and miRNAs.
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