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This protocol details the procedures for transcardial perfusion with HEPES-Sucrose Cutting Solution, fast brain dissection, and flash freezing for the purpose of molecular genetics research.
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01
Gather necessary materials: perfusion pump, cannula, fixation solution (like PBS or paraformaldehyde), and dissection tools.
02
Anesthetize the mouse using an appropriate anesthetic agent.
03
Secure the mouse on a dissection board and ensure a sterile environment.
04
Make a midline incision to expose the chest cavity.
05
Locate the heart and insert the cannula into the left ventricle.
06
Disconnect the right atrium to allow for drainage of the perfusion fluid.
07
Start the perfusion pump and begin by flushing with the fixation solution to clear the blood from the system.
08
Continue perfusing with the fixation solution until the solution runs clear from the right atrium.
09
Once completed, carefully remove the mouse brain for further processing.

Who needs mouse brain perfusion and?

01
Researchers conducting studies in neuroscience, pathology, or histology.
02
Laboratories focused on developing therapies targeting neurological diseases.
03
Institutions performing brain research that requires detailed analysis of brain structures.

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Mouse brain perfusion is a technique used to supply the brain of a mouse with a perfusion solution, often to remove blood and replace it with a fixative or other solution for various experimental purposes, such as studying brain anatomy or pathology.
Researchers and laboratory personnel involved in experiments that require mouse brain perfusion are typically required to file relevant documentation, ensuring compliance with ethical and regulatory guidelines.
Filling out mouse brain perfusion involves documenting the procedures followed, including details such as the type of solution used, the timing of the perfusion, and the methodology undertaken, often following specific institutional protocols.
The purpose of mouse brain perfusion is to prepare the brain for further analysis, such as histological examination, to ensure that the brain tissue is preserved in a specific state for accurate study of its structure and function.
The information that must be reported on mouse brain perfusion includes the species and strain of the mouse, details of the perfusion solution used, the volume of solution administered, the duration of the procedure, and any relevant observations or outcomes.
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