Get the free Mini Protean Tetra Cell - NC-73-12-13(with-corrigendum) - niser ac

CORRIGENDUM REF.: Tender No. NC 73 1213 The tender date for Biology laboratory equipment (Item: Mini. Protean Tetra Cell) is further extended as per the following. 1. Date & Time of Submission: 18.02.2013

How to fill out mini protean tetra cell

How to fill out mini protean tetra cell?

1. Start by assembling the mini protean tetra cell according to the manufacturer's instructions. This usually involves connecting the gel casting module, spacer plates, and lid to form a tight seal.
2. Prepare your gel mixture by following the specific protocol for the experiment you are conducting. This may involve mixing an appropriate concentration of agarose with a suitable running buffer and any desired additives or staining agents.
3. Ensure that the gel solution is completely homogeneous by gently swirling or vortexing it. Avoid introducing any air bubbles as they can interfere with the separation of molecules during electrophoresis.
4. Carefully pour the gel solution into the assembled mini protean tetra cell, ensuring that it evenly fills the space between the spacer plates. Take care not to overfill or underfill the cell, as this can affect the quality of the final results.
5. Insert the comb into the gel solution, making sure it is properly positioned and secured. The comb creates wells where the samples will be loaded for separation.
6. Allow the gel to solidify according to the recommended time in the protocol. This usually involves waiting for around 20-30 minutes for the agarose to set.
7. Once the gel has solidified, carefully remove the comb by gently pulling it straight out of the gel. Take care not to disturb the wells or damage the gel matrix.
8. Add the loading buffer to your samples, mix gently, and carefully load them into the wells using a micropipette. Ensure that each well is loaded with the appropriate sample, keeping track of the order for reference later.
9. Place the lid onto the mini protean tetra cell, ensuring a snug fit to prevent any leakage during electrophoresis.
10. Connect the mini protean tetra cell to a power supply and set the appropriate parameters for your experiment, such as voltage and duration. Start the electrophoresis and monitor the progress as the samples migrate through the gel matrix.

Who needs mini protean tetra cell?

1. Researchers in molecular biology and genetics often use mini protean tetra cells for DNA and RNA analysis, protein separation, and other applications involving electrophoresis.
2. Academic laboratories and educational institutions commonly utilize mini protean tetra cells for teaching purposes and to conduct experiments related to molecular biology and biochemistry.
3. Biotechnology and pharmaceutical companies frequently employ mini protean tetra cells for research and development, quality control, and analysis of biomolecules.
4. Forensic laboratories and paternity testing facilities may use mini protean tetra cells for DNA profiling and other genetic analyses.
5. Clinical laboratories performing diagnostic tests and genetic screening can benefit from mini protean tetra cells to analyze patient samples and identify genetic markers for diseases.

In conclusion, the mini protean tetra cell is a versatile tool widely used by researchers and professionals in various fields of biology and genetics for conducting electrophoresis experiments, analyzing DNA, RNA, and proteins, and advancing scientific knowledge and applications.

For pdfFiller’s FAQs

What is mini protean tetra cell?

The Mini-PROTEAN Tetra Cell is a protein electrophoresis cell used for running mini polyacrylamide gels.

Who is required to file mini protean tetra cell?

Researchers and scientists working in the field of protein analysis are required to use mini protean tetra cell for their experiments.

How to fill out mini protean tetra cell?

To fill out the mini protean tetra cell, one must carefully pour the gel solution into the gel cassette and add the samples for protein analysis.

What is the purpose of mini protean tetra cell?

The purpose of mini protean tetra cell is to separate and analyze proteins based on their size and charge.

What information must be reported on mini protean tetra cell?

The information reported on mini protean tetra cell includes the protein samples, running conditions, and results of the analysis.

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