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NIT LIMITED NO.NC731213 MINI PROTEAN TETRA CELL NATIONAL INSTITUTE OF SCIENCE EDUCATION & RESEARCH INSTITUTE OF PHYSICS CAMPUS, P.O.: SAINT SCHOOL, BHUBANESWAR751 005 Tender Notice No. NIT Limited
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How to fill out mini protean tetra cell

How to fill out mini protean tetra cell:
01
Start by preparing the gel: Cast a polyacrylamide gel using desired concentration and cross-linker ratio. Allow the gel to polymerize completely.
02
Carefully remove the combs from the gel, ensuring not to damage the well slots. Rinse the gel with running buffer to remove any residual unpolymerized acrylamide.
03
Assemble the mini protean tetra cell by attaching the casting base, gasket, and gel assembly onto the cell frame. Ensure that the gel assembly is positioned correctly.
04
Connect the power cables to the electrodes of the mini protean tetra cell. Make sure that the anode (red) and cathode (black) cables are connected to the respective electrode terminals.
05
Fill the upper and lower buffer chambers of the cell with running buffer. Ensure that the electrodes are submerged in the running buffer but do not immerse the gel.
06
Load your samples into the wells of the gel using a micropipette or other suitable loading method. Be careful not to overload the wells and make sure to include appropriate molecular weight markers.
07
Carefully place the lid on the mini protean tetra cell, ensuring that it is properly sealed with no leakage. Double-check the connections and make sure they are secure.
08
Connect the power supply to the mini protean tetra cell and set the desired voltage and running time according to your specific requirements.
09
Start the electrophoresis process by turning on the power supply. Monitor the progress of the run and ensure that the gel is running smoothly without any overheating or abnormalities.
10
Once the electrophoresis is complete, turn off the power supply and carefully remove the lid of the mini protean tetra cell. Take out the gel assembly and proceed with the desired downstream applications such as staining, Western blotting, or imaging.
Who needs mini protean tetra cell:
01
Researchers and scientists working in molecular biology, biochemistry, or related fields who need to perform protein or nucleic acid separation and analysis through electrophoresis.
02
Laboratories involved in protein purification, detection, or characterization, where mini protean tetra cell can facilitate efficient and reliable separation of protein samples.
03
Educational institutions and teaching laboratories that aim to provide hands-on experience to students in gel electrophoresis techniques and experiments.
04
Industrial settings, including pharmaceutical companies, biotechnology firms, and diagnostic laboratories, where mini protean tetra cell can be utilized for routine analysis, quality control, or assay development.
05
Any individual or organization engaged in genetic research, DNA sequencing, or genotyping, where mini protean tetra cell can be employed for DNA fragment separation and analysis.
Note: The mini protean tetra cell is a versatile and widely used system for gel electrophoresis applications, offering convenience, flexibility, and consistent results. Its compact design and user-friendly features make it suitable for various laboratory settings and research purposes.
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What is mini protean tetra cell?
Mini-PROTEAN Tetra cell is a vertical mini gel electrophoresis cell designed for fast, high-throughput protein or nucleic acid separations using precast or handcast gels.
Who is required to file mini protean tetra cell?
Researchers, scientists, or laboratory technicians who are conducting protein or nucleic acid separations and electrophoresis experiments are required to use the mini protean tetra cell.
How to fill out mini protean tetra cell?
To fill out the mini-PROTEAN Tetra cell, simply follow the manufacturer's instructions for preparing and running the gel electrophoresis experiment.
What is the purpose of mini protean tetra cell?
The purpose of the mini-PROTEAN Tetra cell is to separate proteins or nucleic acids based on size and charge through gel electrophoresis for various research and analytical purposes.
What information must be reported on mini protean tetra cell?
The mini-PROTEAN Tetra cell does not require any specific information to be reported as it is a laboratory equipment used for protein or nucleic acid separations.
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