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Chromatography Lab LP Date Objectives: To separate pigments found in markers. To determine the primary colors of pigments. To calculate the Rf value of primary colors. Materials: Filter paper Water
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How to Fill Out Chromatography Lab - USI:
01
Start by gathering all the necessary materials for the chromatography lab, such as chromatography paper, solvent, test substances, and a ruler.
02
Label the chromatography paper with the appropriate identifying information, such as the date, experiment name, and sample names.
03
Use a ruler to draw a clearly marked baseline on the chromatography paper, ensuring it is straight and parallel to the shorter edge of the paper.
04
Apply a small dot or line of the test substance(s) onto the baseline of the chromatography paper using a capillary tube or a thin applicator.
05
Pour a small amount of the appropriate solvent into a container or beaker and carefully suspend the chromatography paper vertically inside the container, ensuring that the test substances do not touch the solvent.
06
Allow the chromatography paper to remain in the container until the solvent front reaches the desired height, usually about 1-2 centimeters from the top edge of the paper.
07
Carefully remove the chromatography paper from the container and immediately mark the solvent front with a pencil or pen.
08
Allow the chromatography paper to thoroughly dry before interpreting the results.
Who Needs Chromatography Lab - USI:
01
Students studying chemistry or related scientific disciplines may need to perform chromatography labs as part of their coursework or assignments.
02
Scientists and researchers in various fields, such as forensic science, pharmaceuticals, biology, or environmental science, may utilize chromatography labs to analyze and separate mixtures of substances.
03
Professionals working in quality control or analytical laboratories may need to conduct chromatography experiments to ensure the purity or composition of certain materials or products.
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