Get the free ADENYLATE CYCLASE - Sigma-Aldrich

Enzymatic Assay of ACETYLATE CYCLE (EC 4.6.1.1) PRINCIPLE: ATP Acetylate Cycle 3 ',5 'cyclic AMP + Pyrophosphate Abbreviations used: ATP Adenosine 5 'Triphosphate 3 ',5 'cyclic AMP 3 ',5 'cyclic Adenosine

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How to fill out adenylate cyclase - sigma-aldrich:

1. Start by carefully reading the instructions provided by sigma-aldrich for the adenylate cyclase product.
2. Make sure you have all the necessary equipment and reagents required for the assay.
3. Prepare a working solution by diluting the adenylate cyclase enzyme provided by sigma-aldrich according to the specified concentration.
4. Add the necessary substrates and activators to the reaction mixture as instructed by sigma-aldrich.
5. Incubate the reaction mixture at the recommended temperature and time specified by sigma-aldrich.
6. After the incubation period, stop the reaction by adding the appropriate stop solution, as provided by sigma-aldrich.
7. Measure the enzymatic activity using the appropriate method recommended by sigma-aldrich, such as colorimetric, fluorescence, or radioisotopic detection.
8. Analyze the obtained data and calculate the adenylate cyclase activity using the formula or software recommended by sigma-aldrich.
9. Finally, interpret and record the results obtained from the adenylate cyclase assay according to the guidelines provided by sigma-aldrich.

Who needs adenylate cyclase - sigma-aldrich:

1. Researchers and scientists in the field of molecular biology, biochemistry, and cellular signaling who are studying the signaling pathways involving adenylate cyclase.
2. Pharmaceutical and biotechnology companies involved in drug discovery and development, particularly those targeting G protein-coupled receptors (GPCRs) and cyclic AMP signaling.
3. Academic institutions and research laboratories that are investigating the role of adenylate cyclase in various physiological and pathological processes such as hormone signaling, neurotransmission, and cancer.

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What is adenylate cyclase - sigma-aldrich?

Adenylate cyclase - sigma-aldrich is an enzyme that catalyzes the conversion of ATP into cyclic AMP.

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Researchers or institutions using adenylate cyclase - sigma-aldrich in their studies may be required to file.

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The form for filing adenylate cyclase - sigma-aldrich may vary depending on the regulatory body overseeing the research.

What is the purpose of adenylate cyclase - sigma-aldrich?

The purpose of adenylate cyclase - sigma-aldrich is to study the effects of cyclic AMP in biological systems.

What information must be reported on adenylate cyclase - sigma-aldrich?

Information such as the source of the enzyme, the quantity used, and the experimental protocols may need to be reported.

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