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Enzymatic Assay of PHOSPHOGLUCOSE ISOMERASE (EC 5.3.1.9) from Bacillus stearothermophilus PRINCIPLE: Fructose Glucose 6Phosphate PGI Glucose 6Phosphate + NADP G6PDH 6Phosphogluconate + NADPH Abbreviations
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How to fill out phosphoglucose isomerase from bacillus:

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Obtain the necessary reagents and equipment for the experiment.
02
Prepare a suitable buffer solution to maintain optimal pH for the enzyme activity.
03
Use a suitable purification technique to isolate phosphoglucose isomerase from bacillus.
04
Determine the concentration of the enzyme using a spectrophotometer or other appropriate method.
05
Set up a reaction mixture containing the purified enzyme, substrate, and cofactors if necessary.
06
Incubate the reaction mixture at the appropriate temperature and monitor the progress of the reaction.
07
Analyze the reaction products using techniques like chromatography or electrophoresis to confirm the activity of the enzyme.
08
Record the experimental data and analyze the results to draw conclusions about the properties and behavior of phosphoglucose isomerase from bacillus.

Who needs phosphoglucose isomerase from bacillus:

01
Researchers studying carbohydrate metabolism may require phosphoglucose isomerase from bacillus to investigate its role in glycolysis and other metabolic pathways.
02
Biotechnologists and biochemists interested in enzyme engineering or protein modification may employ phosphoglucose isomerase from bacillus for various applications.
03
Pharmaceutical companies and drug developers may utilize phosphoglucose isomerase from bacillus for drug screening or development, as it can be a target for enzyme inhibitors.
Note: This is just a general overview and does not cover all potential uses or applications of phosphoglucose isomerase from bacillus. The specific needs for this enzyme may vary depending on the research or industry involved.
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Phosphoglucose isomerase from bacillus is an enzyme that catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate.
Businesses and research institutions working with bacillus strains containing phosphoglucose isomerase are required to file.
The form for reporting phosphoglucose isomerase from bacillus can usually be found on the regulatory agency's website, and it requires information on the source of the enzyme and its intended use.
Phosphoglucose isomerase from bacillus is used in various industrial processes, such as food production and biofuel development.
Information on the source of the enzyme, its specific activity, concentration, and intended use must be reported.
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