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Hypo osmotic Swelling Test HUMAN SPERM VITALITY DIAGNOSTIC (5X 20 TESTS) HOST medium is sterilized by sterile filtration Document reference: FP09 I12 R01 A.12 Update: 08/05/2017 For in vitro diagnostic
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How to fill out hypo-osmotic swelling test

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How to fill out hypo-osmotic swelling test:

01
Prepare the hypotonic solution by dissolving a specific concentration of a solute, such as fructose or sucrose, in a suitable buffer solution.
02
Take a fresh semen sample and place it in a centrifuge tube.
03
Add a predetermined volume of the hypotonic solution to the semen sample.
04
Mix the contents gently and allow them to incubate at a controlled temperature for a specific time period.
05
After incubation, carefully spread a small drop of the sample on a microscope slide and cover it with a coverslip.
06
Observe the slide under a light microscope using low magnification.
07
Count the number of sperm cells showing tail swelling or coiling.
08
Calculate the percentage of sperm cells exhibiting hypo-osmotic swelling by dividing the number of swollen sperm cells by the total number of sperm cells observed and multiplying by 100.

Who needs hypo-osmotic swelling test:

01
Couples undergoing fertility evaluations: The hypo-osmotic swelling test can provide valuable information about the functional ability of sperm cells, helping to determine male fertility potential.
02
Research laboratories: The test is commonly used in scientific studies to assess sperm function, evaluate the effects of various treatments or substances on sperm cells, and investigate mechanisms of infertility.
03
Veterinarians in animal reproduction: The hypo-osmotic swelling test can be utilized to assess the quality of sperm samples in animals, aiding in breeding programs and fertility evaluations for livestock or domestic animals.
04
Andrology clinics: The test is often performed in specialized andrology centers as part of a comprehensive semen analysis to provide additional insights into sperm function and help diagnose certain male reproductive disorders.
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Hypo-osmotic swelling test is a test used to assess the integrity of the sperm cell membrane.
Fertility clinics, research laboratories, and sperm banks are often required to conduct and file hypo-osmotic swelling tests.
Hypo-osmotic swelling test is performed by mixing sperm cells with a hypo-osmotic solution and observing the swelling of sperm tails under a microscope.
The purpose of hypo-osmotic swelling test is to assess the functional status of sperm cell membranes, which can provide information on sperm quality and fertility potential.
The results of hypo-osmotic swelling test typically include the percentage of sperm cells showing swelling of their tails.
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