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Name Section Partner(s) Date SPECTROPHOTOMETRY DETERMINATION OF THE EQUILIBRIUM CONSTANT FOR THE FORMATION OF A COMPLEX ION PRELAB QUERIES 1. What is the oxidation number of iron in Fe SCN+2? 2. Consider
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03
Prepare the cuvette: Clean the cuvette thoroughly to remove any residue or impurities. Fill the cuvette with the prepared sample solution, ensuring it is filled to the desired level.
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Measure the absorbance: Place the cuvette in the spectrophotometer's sample compartment, aligning it with the light beam path. Close the compartment lid and initiate the measurement. Record the absorbance value displayed on the spectrophotometer.
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Repeat the process: If multiple samples need to be analyzed, repeat steps 3 and 4 for each sample. Ensure the cuvette is properly cleaned between measurements to avoid cross-contamination.
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Analyze the data: After obtaining the absorbance values for all the samples, use the appropriate formula or method outlined in the form analysis protocol to determine the desired form quantity or concentration.
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