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Advanced Biology/AP Biology SenegarMitchell NAME DATE PERIOD Floating Leaf Disk Photosynthesis Lab Adapted from Brad Williamson's Leaf Disk Lab (http://www.elbiology.com/labtools/Leafdisk.html) Introduction
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How to fill out floating leaf disk photosynformsis

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How to fill out floating leaf disk photosynthesis:

Start by preparing the leaf disks:

01
Choose healthy and intact leaves from a plant, preferably from the same species.
02
Use a hole punch to cut out small leaf disks from the leaves.
03
Place the leaf disks in a container with water to keep them moist.

Set up the photosynthesis apparatus:

01
Fill a glass or plastic syringe with a bicarbonate solution (usually a mixture of water and sodium bicarbonate).
02
Attach a small hubless syringe needle to the syringe.
03
Fill a beaker or a transparent container with fresh water.
04
Attach the syringe to a ring stand or any other device that allows the syringe to be positioned vertically.

Perform the experiment:

01
Submerge the leaf disks in the water-filled beaker.
02
Gently push the plunger of the syringe to remove the air from the syringe and needle.
03
Keeping the plunger pressed, carefully position the hubless needle on one of the leaf disks.
04
Release the plunger to create a vacuum inside the syringe.
05
The leaf disk should sink initially but will rise as it undergoes photosynthesis.

Measure the rate of photosynthesis:

01
Keep a timer or stopwatch ready.
02
Observe and time how long it takes for the leaf disks to rise to the surface of the water (indicating successful photosynthesis).
03
Repeat the process for multiple leaf disks and record the time taken for each.

Who needs floating leaf disk photosynthesis:

Students studying photosynthesis:

01
Floating leaf disk photosynthesis is a popular experiment in biology classes.
02
It helps students understand the process of photosynthesis and its factors affecting the rate.

Researchers studying plant physiology:

Scientists and researchers may use floating leaf disk photosynthesis to study the efficiency of photosynthesis in different plant species or under varying environmental conditions.

Botanists or plant enthusiasts:

Floating leaf disk photosynthesis can be a useful tool for botanists or plant enthusiasts who want to better understand the photosynthetic capabilities of specific plant species.
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