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WHO Global Foodborne Infections Network (formerly WHO Global Salisbury) “A WHO network building capacity to detect, control and prevent foodborne and other enteric infections from farm to table

How to fill out susceptibility testing of enterobacteriaceae

How to fill out susceptibility testing of enterobacteriaceae:

1. Obtain a culture of the isolate: First, you need to obtain a pure culture of the enterobacteriaceae isolate that you want to test for susceptibility. This can be achieved by streaking the isolate on an appropriate culture medium and incubating it at the appropriate conditions for growth.
2. Prepare inoculum: Once you have a pure culture, prepare an inoculum by suspending several colonies of the isolate in sterile saline or a suitable broth. Adjust the turbidity of the suspension to match the turbidity of a McFarland standard tube, which represents a standard concentration of bacteria.
3. Set up the test panel: Depending on the available resources and the desired antimicrobial agents to be tested, select an appropriate susceptibility testing method such as disk diffusion, E-test, or broth microdilution. Prepare the test panel by placing the antimicrobial disks or adding the antimicrobial agents to the microdilution wells according to the manufacturer's instructions.
4. Inoculate the plates: Using a sterile swab or loop, streak the prepared inoculum onto the surface of the agar plates containing the antimicrobial agents. Ensure even distribution of the inoculum across the plate.
5. Incubate the plates: Incubate the plates at the appropriate temperature and conditions for the enterobacteriaceae isolate being tested. This allows the bacteria to grow and the antimicrobial agents to inhibit their growth, indicating their susceptibility or resistance.
6. Measure the zones of inhibition: After incubation, measure the zones of inhibition around the antimicrobial disks or in the microdilution wells. The size of the zones determines the susceptibility or resistance of the isolate to the tested antimicrobial agents. Use interpretive guidelines provided by regulatory agencies or other reliable sources to determine the susceptibility category for each antimicrobial agent tested.

Who needs susceptibility testing of enterobacteriaceae?

1. Healthcare professionals: Susceptibility testing of enterobacteriaceae is primarily performed by healthcare professionals, including clinical microbiologists and infectious disease specialists. They use this information to guide the appropriate selection of antimicrobial therapy for patients infected with enterobacteriaceae.
2. Hospitals and diagnostic laboratories: Hospitals and diagnostic laboratories perform susceptibility testing on enterobacteriaceae isolates to monitor the antimicrobial resistance patterns of these organisms within their patient population. This helps guide local treatment protocols and monitor the emergence of multi-drug resistant strains.
3. Researchers: Researchers studying antimicrobial resistance, mechanisms of action, or new antimicrobial agents may also perform susceptibility testing on enterobacteriaceae to evaluate the efficacy of their interventions or to understand the resistance mechanisms employed by these bacteria.

Note: Susceptibility testing of enterobacteriaceae is crucial in the ongoing fight against antibiotic resistance, as it helps inform treatment strategies and the development of new antimicrobial agents.

For pdfFiller’s FAQs

What is susceptibility testing of enterobacteriaceae?

Susceptibility testing of enterobacteriaceae is a laboratory procedure used to determine the effectiveness of antibiotics against bacteria belonging to the Enterobacteriaceae family.

Who is required to file susceptibility testing of enterobacteriaceae?

Healthcare facilities and laboratories are required to perform and file susceptibility testing of enterobacteriaceae.

How to fill out susceptibility testing of enterobacteriaceae?

Susceptibility testing of enterobacteriaceae is filled out by performing antibiotic susceptibility tests on bacterial isolates and recording the results in a standardized format.

What is the purpose of susceptibility testing of enterobacteriaceae?

The purpose of susceptibility testing of enterobacteriaceae is to guide healthcare providers in choosing the most appropriate antibiotic treatment for infections caused by Enterobacteriaceae bacteria.

What information must be reported on susceptibility testing of enterobacteriaceae?

The results of antibiotic susceptibility testing, including the name of the antibiotics tested, the minimum inhibitory concentration (MIC) values, and the interpretation of the results must be reported on susceptibility testing of enterobacteriaceae.

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