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The resolution of a specimen viewed through a microscope can be increased by changing the objective lens. The objective lenses are the lenses that protrude downward over the specimen. Grasp the nose piece. The nose piece is the platform on the microscope to which the three or four objective lenses are attached.
As discussed above, the primary factor in determining resolution is the objective numerical aperture, but resolution is also dependent upon the type of specimen, coherence of illumination, degree of aberration correction, and other factors such as contrast enhancing methodology either in the optical system of the
The resolution of a specimen viewed through a microscope can be increased by changing the objective lens. The objective lenses are the lenses that protrude downward over the specimen. Grasp the nose piece. The nose piece is the platform on the microscope to which the three or four objective lenses are attached.
The resolution of the light microscope cannot be smaller than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 Am), the objects which are, at most, about 0.2 Am. Below this point, light microscope is not useful, as wavelength smaller than 400 nm is needed.
Another method of improving microscope resolution is to increase the refractive index between the objective lens and the specimen. Microscope resolution is the most important determinant of how well a microscope will perform and is determined by the numerical aperture and light wavelength.
A simple answer is that resolution of an optical system is its ability to separate two closely spaced objects. Resolution In general as the magnification increases the resolution increases, however, this much more complicated than it seems.
The maximum magnification of light microscopes is usually ×1500, and their maximum resolution is 200 nm, due to the wavelength of light. An advantage of the light microscope is that it can be used to view a variety of samples, including whole living organisms or sections of larger plants and animals.
The resolution of the light microscope cannot be small than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 Am), the objects which are, at most, about 0.2 Am.
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