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The initial document that I needed was insufficient on the site (the word SAMPLE was written across the document multiple times per page). And, although it took several hours, PDFfiller was able to upload the necessary document. Problem solved.
2015-09-04
i love it but i have a few things i don't know how to do like if I download a pdf and want to make more space for a word i don't know how to do that. you cant just hit the space bar and move it.
2018-06-30
An easy to use program, great features, that allows you to complete and edit PDF forms. I'm no computer expert, but found this program straight forward to follow. Makes completing forms online so much easier than printing onto paper and filling out the long way. Would highly recommend it.
2018-08-19
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Yesterday, I needed assistance with a PDF, which, when it was submitted to PDFFiller, would change the computer bar and make it into numbers. Since I needed this document in its original form, I contacted their customer service, and Kara helped me. Kara went out of her way to make sure my problem was resolved. It was really nice to talk to a real human instead of a bot, and that human being was so kind and able. My problem is not completely resolved, but she taught me how to be able to fill the PDF without losing the bar and eventually make a template, which is what I need to do. Awesome team-mate, you got there, PDF Filler!
2024-02-27
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Nothing. I had a good experience using this software
2023-03-01
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2022-06-01
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2021-10-05
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I have been satisfied with the subscription and have used it with my work, however I am retiring the end of September and will no longer need this subscription
2021-08-23
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Templates and ability to locate my docs 10
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No spell check available and offers no ability to number or set bullets
Recommendations to others considering the product:
TRY the free version first
What problems are you solving with the product? What benefits have you realized?
Professional-looking documents rather than handwriting
2021-07-15
Polish Columns Resolution Feature
The Polish Columns Resolution feature streamlines and enhances your data organization, making it easier for you to manage and access necessary information. This tool refines your columns, ensuring clarity and precision in your data presentation.
Key Features
Automated data cleaning to remove duplicates and inconsistencies
Intuitive interface for easy navigation and usage
Customizable settings to fit your specific data needs
Integration with existing software for seamless operation
Real-time updates to keep your data current
Potential Use Cases and Benefits
Improve data accuracy in reports and analyses
Enhance team collaboration through clearer data structure
Boost productivity by reducing time spent on data management
Support better decision-making with reliable data at your fingertips
Facilitate data migrations by organizing and prepping data effectively
By implementing the Polish Columns Resolution feature, you tackle common data issues head-on. It reduces confusion, saves time, and provides a clear path to managing your data effectively. You can focus on what matters most—achieving your goals and driving your business success.
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How does changing salt concentrations change interactions in the chromatography column?
Salting out will release, or elute, your protein from the column. This technique uses a high salt concentration solution. The salt solution will out-compete the protein in binding to the column. ... Proteins with weaker ionic interactions will elute at a lower salt, so you will often want to elute with a salt gradient.
How does salt interact with proteins?
Charged proteins bind to the oppositely charged resins via electrostatic interactions in IEC. Salts, due to its nonspecific effects, modulate binding affinity (binding constant) of the protein. While any salts can be used as a source of ions, NaCl is typically used.
How does salt affect proteins?
The solubility of proteins usually increases slightly in the presence of salt, referred to as “salting in”. However, at high concentrations of salt, the solubility of the proteins drop sharply and proteins can precipitate out, referred to as “salting out”.
Why do proteins precipitate at high salt concentrations?
It relies on the principle that most proteins are less soluble in solutions of high salt concentrations because the addition of salt ions shield proteins with multi-ion charges. Those charges help protein molecules interact, aggregate, and precipitate. ... However, the salt can pose a problem to the purity of protein.
Why does salt increase hydrophobic interactions?
The high salt concentration enhances the interaction between the hydrophobic components of the sample and the chromatography medium, and the lower salt concentration weakens the interaction. These interactions are a result of the presence of side chains of hydrophobic amino acids.
How does a HIC column work?
How Does HIC Work? In a nutshell, HIC (also known as 'salting out') separates protein molecules using the properties of hydrophobicity. In this method, proteins containing both hydrophilic and hydrophobic regions are applied to an HIC column under high salt buffer conditions.
What is HIC used for?
Hydrophobic interaction chromatography (HIC) is a valuable tool used in protein purification applications. HIC is used in the purification of proteins over a broad range of scales-in both analytical and preparatory scale applications.
What is hydrophobic interaction chromatography?
Hydrophobic Interaction Chromatography (HIC)[edit] Hydrophobic Interaction Chromatography is a separation technique that uses the properties of hydrophobicity to separate proteins from one another. In this type of chromatography, hydrophobic groups such as phenol, octal, or butyl, are attached to the stationary column.
Why is GAP hydrophobic?
GAP has several stretches of hydrophobic amino acids, which results in the total protein being very hydrophobic. When the supernatant, rich in GAP, is passed over a HIC column in a highly salty buffer (Binding Buffer), the hydrophobic regions of the GAP stick to the HIC beads.
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