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How is mRNA stability measured?
One of the simplest techniques of measuring mRNA stability is by inhibiting transcription in vivo with transcription inhibitors and measuring the mRNA kinetics. Antimycin D is a transcription inhibitor which intercalates into DNA.
How are transcription factors regulated?
Transcription factors include a wide number of proteins, excluding RNA polymerase, that initiate and regulate the transcription of genes. ... Other transcription factors bind to regulatory sequences, such as enhancer sequences, and can either stimulate or repress transcription of the related gene.
How is the initiation of transcription regulated?
Regulatory landscape Transcriptional initiation, termination and regulation are mediated by DNA looping which brings together promoters, enhancers, transcription factors and RNA processing factors to accurately regulate gene expression. ... The configuration of the genome is essential for enhancer-promoter proximity.
What is mRNA stability?
mRNA levels in living cells represent the balance between production (transcription) and decay (mRNA degradation). mRNA stability, as an important factor in the control of gene expression, only depends on degradation rates of mRNA and does not relate to the steady-state levels of mRNA.
What are three mechanisms by which transcription factors regulate eukaryotic gene expression?
However, unlike prokaryotic cells, the eukaryotic RNA polymerase requires other proteins, or transcription factors, to facilitate transcription initiation. Transcription factors are proteins that bind to the promoter sequence and other regulatory sequences to control the transcription of the target gene.
What is a way or some ways in which bacteria regulate transcription?
Bacteria have specific regulatory molecules that control whether a particular gene will be transcribed into mRNA. Often, these molecules act by binding to DNA near the gene and helping or blocking the transcription enzyme, RNA polymerase.
How is gene expression regulated in eukaryotes?
Gene expression in eukaryotic cells is regulated by repressors as well as by transcriptional activators. Like their prokaryotic counterparts, eukaryotic repressors bind to specific DNA sequences and inhibit transcription. ... Other repressors compete with activators for binding to specific regulatory sequences.
How are transcription factors activated?
Transcription Factors. Transcription factors are proteins possessing domains that bind to the DNA of promoter or enhancer regions of specific genes. ... They typically do so by acting on promoters or enhancers to activate or repress the transcription of specific genes.
What is the difference between general transcription factors and regulatory transcription factors?
General transcription factors are essential for any transcription for all genes while regulatory transcription factors regulate transcription of specific genes. ... General transcription factors only regulate the housekeeping genes while regulatory transcription factors control all the other genes.
What is mRNA stability control?
mRNA stability and the control of gene expression: implications for human disease. ... The stability of a given mRNA transcript is determined by the presence of sequences within an mRNA known as cis-elements, which can be bound by trans-acting RNA-binding proteins to inhibit or enhance mRNA decay.
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