Analyze Number Resolution Gratuito

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Resolution and Separation Factor, Retention Factor, Number of Theoretical Plates. ... The separation factor is defined as the ratio of the retention factors (k), as shown in equation (3).
1:47 7:52 Suggested clip Resolution of chromatography — YouTubeYouTubeStart of suggested client of suggested clip Resolution of chromatography — YouTube
1:19 7:52 Suggested clip Resolution of chromatography — YouTubeYouTubeStart of suggested client of suggested clip Resolution of chromatography — YouTube
The resolution of an elation is a quantitative measure of how well two elation peaks can be differentiated in a chromatographic separation. It is defined as the difference in retention times between the two peaks, divided by the combined widths of the elation peaks.
Equation (1) indicates that the resolution is the difference between peak retention times divided by the average peak width. In a peak with Gaussian distribution, the peak width is W = 4 (where is the standard deviation) and the peak FHM is W0.5h = 2.354.
Definition: Tailing factor The tailing factor is a measure of peak tailing. It is defined as the distance from the front slope of the peak to the back slope divided by twice the distance from the center line of the peak to the front slope, with all measurements made at 5% of the maximum peak height.
f) The retention factor (k) is the ratio of the amount of analyte in the stationary phase to the amount in the mobile phase. It is generally calculated by k' = (tR — TM)/TM = tRy'/TM. g) The selectivity factor () of a column for two analytes (A eluting before B) is given by = KB/A = k'(B)/k'(A) = tRy'(B)/tR'(A).
The selectivity (or separation) factor () is the ability of the chromatographic system to 'chemically' distinguish between sample components. It is usually measured as a ratio of the retention (capacity) factors (k) of the two peaks in question and can be visualized as the distance between the spices of the two peaks.
The capacity factor (also called “capacity ratio”) is symbolized by k' (USP terminology) or k (IUPAC/ASTM terminology). It is a measure of the retention of a peak that is independent of column geometry or mobile phase flow rate. The capacity factor is calculated as: k' = (tR — t0)/t0.
The retention factor and the retention time can be used to describe the chromatographic behavior of a sample component in a column. The retention factor is a relative value (dimensionless). ... k = 1 means that the component spends just as much time in the stationary phase than in the mobile phase.

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