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I'm loving the versatility of this application and I'm really glad that I bought it. I've used it in a variety of situations and I especially like that I can upload a non-pdf document and choose the output that works best for my needs.
2015-09-30
It has been a tremendous help in my business, saving time and paper trails, establishing forms. I would recommend this to everyone! I have been very pleased with how easy it is to use! 5 Star all the way.
2016-04-25
Great for starting my transportation business. PDF filler help me to search and make documents that I needed for inspections, inventories, vehicle maintenance and more..
2019-06-04
Very easy to use
I am excited to learn all the features the software has to offer.
I am so happy i can take current PDF files and turn them into filliable files. This has been very helpful as i started this position and can't always find fillable files.
2017-11-14
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I encounter a lot of reasons to amend, annotate, merge, separate, and complete PDF documents. I used the free version of pdfFiller for a while and realized that I used it so much and liked using it so much that I actually paid for the upgraded version. I have been using pdfFiller for about a year and it is a go-to part of my toolbox for document management. In fact, I just grabbed a couple of model forms from my trade association and (with permission) stripped off their information and inserted my firm info and logo. In 15 minutes I had professional forms tailored to my business - I cannot even estimate how much that saved me in time and money. pdfFiller makes me look good, so I am giving them a review to make them look good - it's what I can Good Business quid-pro-quo.
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Nothing is perfect, and while I gave pdfFiller the highest rating I could, it too is not perfect. I have found that scrolling through documents I have edited rather than being able to create folders to store my completed documents is slower than I want. I come from a Windows environment and have become spoiled by the Windows' file structure. Still, this is probably more about me than about pdfFiller.
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I annotate, merge, duplicate, and complete more pdf forms than I like. Most are government or bureaucratic forms and they insist upon too much information. It helps to have forms done one and stored on pdfFIller that I can access to update the dates or other data and resubmit.
2022-05-19
Kara was VERY helpful
Kara was very helpful, especially in suggesting we do a Zoom screen share, which made some problems I was having with navigation and document publishing much clearer. She should get a raise.
2021-11-26
Payment issue dealt with swiftly
After both my cards being declined to register my subscription, I went onto the online support chat. Kara was super helpful and quick to deal with my issue - very professional and friendly. Kara was very generous in giving me three free days and advised within that time to try my payment again after 24 hours. Thank you Kara for taking the stress away and resolving my issue fast. Morven
2021-09-16
This program is such a life saver
This program is such a life saver, very easy to use, now people I send documents will be able to read the document, also allows you to sign a document as well. 10 out of a 10.
2021-05-16
Well-designed and functional, user-friendly
I use pdfFiller almost every day for my business proofreading for court reporters.
It's been invaluable, whether I need to save a PDF in a different format (.docx, .xlsx, etc.), combine hundreds of pages or PDFs into one PDF, or quickly edit a PDF.
I searched long and hard to find this program, which unlike other PDF programs I tried (including Adobe), consistently delivers on what I need it to do, is well-designed, functional, and user-friendly.
And on top of all that, support responds almost magically quickly to questions or issues, like I had today, which in a deadline-driven business like mine is invaluable.
2025-03-27
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How much DNA do you add to transformation?
High Efficiency Transformation Protocol Add 1 pg-100 NG of plasmid DNA (1-5 SL) to cells and mix without voting.
How much DNA do you need for transformation?
For successful chemical transformation, 50100 SL of competent cells and 110 NG of DNA are recommended. When a ligation mixture is used as the transforming DNA (often 15 All is sufficient), purification prior to chemical transformation is generally not required.
What is required for transformation?
The process of gene transfer by transformation does not require a living donor cell but only requires the presence of persistent DNA in the environment. The prerequisite for bacteria to undergo transformation is its ability to take up free, extracellular genetic material. Such bacteria are termed as competent cells.
How much plasmid do I need for transformation?
Most recent answer. When you get at least 100 CFU from a transformation made with 0.1 ng of circular plasmid (ideally pUC18 or pUC19). This level of competency (106 CFU/kg of DNA) is the lower limit required for efficient cloning purpose.
What is a good transformation efficiency?
A good rule to follow is this: if your efficiency is equal to or less than 5 × 107 CFU/kg DNA, use these cells for plasmid transformations. If your efficiency is greater than 5 × 107 (ideally 1 × 108 or higher), use these cells for ligation and other assembly reaction transformations.
How much DNA do you add to electroporating?
Once DNA is added to the cells, electroporating can be carried out immediately. It is not necessary to incubate DNA with cells. The maximum recommended volume of a DNA solution to be added is 2.5 SL.
How do you do electroporating?
Step 1 : Prepare cells. Prepare cells by suspending in electroporating buffer. Step 2 : Apply electrical pulse. Apply electrical pulse to cells in the presence of specialized buffer and nucleic acids. Step 3 : Return cells to growing conditions. Step 4 : Assay cells.
Which current is used in electroporating?
Abbreviation for alternating current which is an oscillating electrophoretic current in which an electrical current rises to a maximum point in one direction and falls to zero and then rises in the opposite direction and then repeats. Refers to the use of AC current to align cells prior to electrocution.
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