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This document serves as a self-assessment tool for evaluating understanding and application of antimicrobial susceptibility testing, including Kirby-Bauer methods, quality control, and interpretation
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How to fill out antimicrobial susceptibility testing self-assessment

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How to fill out Antimicrobial Susceptibility Testing Self-Assessment

01
Review the provided guidelines on antimicrobial susceptibility testing.
02
Gather all necessary data and resources related to your organization's antibiotic use.
03
Begin with section 1 of the self-assessment, answering each question based on your current practices.
04
Continue to sections 2 and 3, ensuring accuracy in your responses by consulting relevant laboratory results and protocols.
05
Check for any specific requirements or benchmarks that need to be met in each section.
06
Once completed, review your answers for clarity and completeness before submission.
07
Submit the self-assessment by the given deadline and keep a copy for your records.

Who needs Antimicrobial Susceptibility Testing Self-Assessment?

01
Healthcare facilities involved in the diagnosis and treatment of bacterial infections.
02
Laboratories conducting susceptibility testing on microbial pathogens.
03
Infection control teams aiming to manage antibiotic use effectively.
04
Pharmacists and clinicians involved in antibiotic stewardship programs.
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Susceptible means they can't grow if the drug is present. This means the antibiotic is effective against the bacteria. Resistant means the bacteria can grow even if the drug is present. This is a sign of an ineffective antibiotic.
CLSI M100 is the gold standard for antimicrobial susceptibility testing, relied upon by laboratories worldwide to guide life-saving clinical decisions.
A sensitive result indicates the bacteria will die when it is exposed to the antibiotic. An intermediate result indicates the antibiotic must be used in combination with another antibiotic to clear the infection. A resistant result indicates the antibiotic does not the bacteria.
A “susceptible” result indicates that the patient's organism should respond to therapy with that antibiotic using the dosage recommended normally for that type of infection and species [13, 20].
Interpretation of MIC The determined MIC value must be compared with MIC clinical breakpoints to assess whether the strain is susceptible or resistant to the antibiotic. Evaluation of antibiotic resistance based on the MIC value does not mean the identification of the resistance mechanism.
The method is based on placing different antibiotic-impregnated disks on previously inoculated agar with bacterial suspension. The antibiotic diffuses radially outward through the agar medium, producing an antibiotic concentration gradient.

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Antimicrobial Susceptibility Testing Self-Assessment is a process where laboratories evaluate their own practices and performance in determining how effective antimicrobial agents are against specific pathogens, ensuring adherence to standards and improving the accuracy of testing.
All laboratories that perform antimicrobial susceptibility testing on microorganisms are generally required to file Antimicrobial Susceptibility Testing Self-Assessments as part of quality assurance and regulatory compliance.
To fill out the Antimicrobial Susceptibility Testing Self-Assessment, laboratories should review their testing methodologies, quality control measures, interpretive criteria, and documentation practices, and then complete the assessment form with relevant details and findings.
The purpose of Antimicrobial Susceptibility Testing Self-Assessment is to ensure that laboratories maintain high standards in testing practices, improve patient care through accurate antimicrobial susceptibility results, and comply with regulatory requirements.
The information that must be reported includes the laboratory's testing methods, quality control results, adherence to guidelines, staff training records, and any corrective actions taken if discrepancies were found during the self-assessment process.
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