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This document provides guidelines from the University of Pennsylvania's Institutional Animal Care and Use Committee regarding the use of carbon dioxide for the euthanasia of laboratory rodents, focusing
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How to fill out iacuc guideline carbon dioxide
How to fill out IACUC Guideline Carbon Dioxide Euthanasia of Rodents
01
Start by preparing the euthanasia chamber with proper ventilation.
02
Ensure that the chamber size is appropriate for the number of rodents being euthanized.
03
Calibrate the carbon dioxide (CO2) gas delivery system to provide a controlled flow.
04
Place the rodents in the chamber, making sure not to overcrowd.
05
Begin the gas flow slowly to reduce stress on the animals.
06
Monitor the rodents closely for signs of loss of consciousness (e.g., lack of movement).
07
Allow the rodents to remain in the chamber until cessation of breathing is confirmed.
08
Follow up with secondary euthanasia verification methods if necessary, such as cervical dislocation or confirming cardiac arrest.
Who needs IACUC Guideline Carbon Dioxide Euthanasia of Rodents?
01
Researchers conducting animal studies involving rodents.
02
Veterinary professionals involved in rodent care.
03
Laboratories and facilities that perform animal research.
04
Institutions or organizations that require adherence to ethical standards in animal research.
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People Also Ask about
Is CO2 euthanasia painful for mice?
It's as quick as bashing their skulls in but not as messy and is generally painless since you're basically dislocating their cervical spine before they can even feel the pain. That being said, CO2 is the more commonly practiced method because it's easier to perform on large groups of rodents.
What is the IACUC required to do regarding euthanasia methods?
Large Mouse Cage (10½" wide x 19" deep x 6" high): 6.0 – 14.0 liters per minute. c. Rat Cage (10½" wide x 19" deep x 8" high): 8.0 – 18.0 liters per minute. Once the rodent appears unconscious (i.e., recumbent, absence of purposeful movement) you may increase the flow rate of CO2.
How much CO2 does it take to euthanize a rat?
To euthanize mice humanely by carbon dioxide inhalation, one should use a gradual fill method, ensure that mice are in a familiar and non-overcrowded environment, and maintain equipment regularly.
Which of the following statements is true about the use of carbon dioxide for euthanasia?
The true statement about carbon dioxide for euthanasia is that it is acceptable under certain conditions where distress can be minimized. Proper use involves careful monitoring to avoid causing panic or suffering in the animals.
What are the AVMA guidelines for CO2 euthanasia?
The following statement is recommended for inclusion in an Animal Use Protocol for any species where CO2 euthanasia is to be used: “Animals will be euthanized using a 30-70% per minute displacement of chamber air with compressed CO2.
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What is IACUC Guideline Carbon Dioxide Euthanasia of Rodents?
The IACUC Guideline for Carbon Dioxide Euthanasia of Rodents provides standards and procedures for the humane euthanization of rodents using carbon dioxide, ensuring compliance with ethical practices and regulations.
Who is required to file IACUC Guideline Carbon Dioxide Euthanasia of Rodents?
Researchers and institutions that conduct studies involving the euthanasia of rodents are required to file the IACUC Guideline for Carbon Dioxide Euthanasia as part of their animal care and use protocols.
How to fill out IACUC Guideline Carbon Dioxide Euthanasia of Rodents?
To fill out the IACUC Guideline for Carbon Dioxide Euthanasia, researchers should provide detailed information on the methodology, species involved, dosage, equipment to be used, and the justification for using carbon dioxide as an euthanasia method.
What is the purpose of IACUC Guideline Carbon Dioxide Euthanasia of Rodents?
The purpose of the IACUC Guideline is to ensure that the euthanasia of rodents is performed humanely, effectively, and in accordance with legal and ethical standards, minimizing suffering and distress.
What information must be reported on IACUC Guideline Carbon Dioxide Euthanasia of Rodents?
The information that must be reported includes the species and number of rodents, the method of euthanasia, the administration process, monitoring procedures, and any potential distress or pain assessment during the euthanasia process.
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