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Antihistamine C (Egg) ELISA For the quantitative and qualitative detection of antibodies against histones in serum. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size:

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How to fill out antihistone c igg elisa

How to fill out antihistone c igg elisa:

01

Start by gathering all the necessary materials for the assay, including the antihistone c IgG ELISA kit, microplate, micropipettes, and samples.

02

Prepare the microplate by adding the provided antihistone c antigen to each well, following the manufacturer's instructions.

03

Add the samples into separate wells, making sure to label each well correctly.

04

Incubate the microplate at the designated temperature and duration, as specified in the kit instructions.

05

After the incubation period, carefully remove the liquid from each well by gentle washing or aspiration.

06

Add the enzyme-conjugated antihistone c IgG antibody to each well, ensuring complete coverage of the antigens.

07

Incubate the microplate again, allowing the antibody to bind to any specific IgG present in the samples.

08

Wash or aspirate the wells to remove any unbound antibodies.

09

Add the substrate solution to each well, which will initiate a color change reaction if the antihistone c IgG is present.

10

Allow the color development to occur for the recommended time, as suggested by the kit instructions.

11

Stop the reaction by adding the stop solution, which will halt the color change process.

12

Measure the absorbance or optical density of each well using a microplate reader at the appropriate wavelength specified by the kit instructions.

13

Compare the absorbance values of the samples to the provided standard curve or control values to determine the concentration or presence of antihistone c IgG in each sample.

Who needs antihistone c IgG ELISA:

01

Individuals with suspected autoimmune diseases: Antihistone c IgG ELISA may be used to detect the presence of antihistone c IgG antibodies in patients with suspected autoimmune diseases related to the forming of immune complexes involving histones.

02

Diagnosing drug-induced lupus erythematosus: Antihistone c IgG antibodies are often associated with drug-induced lupus erythematosus (DIL), a condition triggered by certain medications. Antihistone c IgG ELISA can help confirm the diagnosis in such cases.

03

Monitoring treatment response: Antihistone c IgG ELISA can be used to monitor the effectiveness of treatment in individuals diagnosed with conditions related to antihistone c IgG antibodies, such as lupus or DIL. Regular testing can help gauge the progress of the treatment and make necessary adjustments if needed.

Note: It is important to consult with a healthcare professional or refer to the specific guidelines provided by the manufacturer for accurate and proper usage of the antihistone c IgG ELISA kit.

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What is antihistone c igg elisa?

Antihistone C IgG ELISA is a laboratory test that detects antibodies in the blood against histones, which are proteins that bind to DNA.

Who is required to file antihistone c igg elisa?

Medical professionals and researchers may be required to perform and report antihistone C IgG ELISA tests.

How to fill out antihistone c igg elisa?

To fill out antihistone C IgG ELISA, follow the specific instructions provided by the laboratory or healthcare provider administering the test.

What is the purpose of antihistone c igg elisa?

The purpose of antihistone C IgG ELISA is to diagnose autoimmune diseases, specifically those involving antibodies against histones.

What information must be reported on antihistone c igg elisa?

The results of the antihistone C IgG ELISA test, including antibody levels and any relevant clinical information, must be reported.

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