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Spreadsheet Software for Thermodynamic Melting Point Prediction of Oligonucleotide Hybridization with and without Mismatches BioTechniques 27 1218-1224 December 1999 Ekkehard Sch tz and Nicolas von Ahsen Georg-August-University G ttingen Germany ABSTRACT The use of thermodynamic parameters for the calculation of oligonucleotide duplex stability provides the best estimates of used for evidence-based design of molecular biological experiments in wh...
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