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DOCUMENT NO: 128RAT.TO DATE: PAGE 1 OF 10 28DAY TOXICITY OF IN RATS SPONSOR: Toxicology & Pharmacology Branch Developmental Therapeutics Program Division of Cancer Treatment National Cancer Institute
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Start by gathering all the necessary information and materials for the study.
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Prepare the test substance and control substance according to the specified guidelines.
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Administer the substances to the test subjects on a daily basis for a period of 28 days.
04
Observe and record any changes or effects on the test subjects throughout the study.
05
Perform regular measurements and assessments to evaluate the toxicity of the substances.
06
At the end of the study, analyze the data and draw conclusions regarding the 28-day toxicity of the substance.

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Pharmaceutical companies and researchers conducting preclinical studies on new drugs.
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Regulatory authorities evaluating the safety of certain substances or chemicals.
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Environmental agencies assessing the potential toxicity of pollutants.
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Toxicology laboratories performing studies to understand the effects of substances on living organisms.
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The 28-day toxicity study is a type of safety assessment conducted to evaluate the potential harmful effects of a substance on living organisms when exposed for a period of 28 days.
Manufacturers, importers, or distributors of the substance are required to file the 28-day toxicity study.
The 28-day toxicity study should be completed according to standard protocols and guidelines set by regulatory authorities.
The purpose of the 28-day toxicity study is to assess the short-term effects of a substance on living organisms and to determine any potential risks to human health or the environment.
The 28-day toxicity study should include detailed information on the test substance, test methods, results, and conclusions drawn from the study.
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