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Aura mine AFB Stain SOP Author: Anne Morris Document Number: Pro67C13Effective (or Post) Date: 10 Feb 09 Document Origin; Site Laboratory Company: BiotechnologySMILE Approved by: Peggy Coulter Review
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How to fill out auramine stain sop

01
Prepare the auramine staining solution according to the specified concentration mentioned in the Standard Operating Procedure (SOP).
02
Label the slides or containers properly to identify the samples.
03
Ensure that all the required safety precautions are followed, such as wearing gloves and working in a properly ventilated area.
04
Place the slides or samples on a staining rack or holder.
05
Immerse the slides or samples in the auramine staining solution for the specified duration mentioned in the SOP.
06
After the staining period, gently lift the slides or samples from the staining solution.
07
Rinse the slides or samples with distilled water to remove excess stain.
08
Place the slides or samples in a container with appropriate mounting medium.
09
Cover the slides or containers with coverslips.
10
Allow the slides or containers to dry completely before examining under a fluorescence microscope or any required analysis equipment.

Who needs auramine stain sop?

01
Medical laboratories performing tuberculosis testing
02
Research institutions studying tuberculosis
03
Clinical facilities investigating potential tuberculosis cases
04
Public health agencies monitoring tuberculosis outbreaks

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Auramine stain SOP (Standard Operating Procedure) is a documented process that outlines the steps required to perform the auramine staining technique, which is used primarily in microbiology to visualize the presence of mycobacteria in clinical samples.
Laboratories and organizations that perform auramine staining as part of diagnostic testing for diseases like tuberculosis are required to file the auramine stain SOP.
Filling out the auramine stain SOP typically involves detailing the procedures, materials needed, safety precautions, quality control measures, and any relevant regulations or standards that must be adhered to.
The purpose of the auramine stain SOP is to ensure consistency, accuracy, and safety in performing the staining technique, thereby producing reliable results for the detection of mycobacteria.
The auramine stain SOP must report information such as the procedure steps, reagents used, equipment needed, quality control results, and any deviations from the protocol.
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