Get the free EpiMark Hot Start Taq DNA Polymerase
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240 County Road
Ipswich, MA 019382723Tel 9789275054
Fax 9789211350www.neb.com
info@neb.comNew England Biol abs
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01
Thaw Epimark Hot Start Taq DNA Polymerase, Reaction Buffer, and 25 mM MgCl2 on ice.
02
Prepare the reaction mix by combining the following components in a PCR tube:
03
- X μL Epimark Hot Start Taq DNA Polymerase
04
- X μL Reaction Buffer
05
- X μL 25 mM MgCl2
06
- X μL Template DNA
07
- X μL Forward Primer (X μM final concentration)
08
- X μL Reverse Primer (X μM final concentration)
09
- X μL dNTPs (X mM each final concentration)
10
- X μL Nuclease-free Water
11
Gently mix the reaction mix by pipetting up and down.
12
Centrifuge the PCR tube briefly to collect all the liquid at the bottom.
13
Place the PCR tube in a thermal cycler and perform the following cycling conditions:
14
- Initial denaturation: X°C for X minutes
15
- Denaturation: X°C for X seconds
16
- Annealing: X°C for X seconds
17
- Extension: X°C for X seconds
18
- Final extension: X°C for X minutes
19
- Hold: X°C indefinitely
20
After the cycling is complete, the PCR product can be used for downstream applications.
Who needs epimark hot start taq?
01
Epimark Hot Start Taq is particularly useful for researchers working with sensitive DNA templates or when specific amplification of a target sequence is required. It is beneficial in applications such as PCR, RT-PCR, SNP genotyping, and mutational analysis.
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What is epimark hot start taq?
Epimark Hot Start Taq is a specialized DNA polymerase enzyme used for PCR reactions that incorporates an antibody-based, hot start polymerase activation method.
Who is required to file epimark hot start taq?
Epimark Hot Start Taq is typically used in molecular biology laboratories by researchers and scientists.
How to fill out epimark hot start taq?
To use Epimark Hot Start Taq, follow the manufacturer's protocol and guidelines for PCR reactions.
What is the purpose of epimark hot start taq?
The purpose of Epimark Hot Start Taq is to improve the specificity and sensitivity of PCR reactions by preventing non-specific amplification.
What information must be reported on epimark hot start taq?
The information reported with Epimark Hot Start Taq includes the specific DNA template, primers, nucleotides, and reaction conditions used.
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