Get the free NEBNext Ultra II Directional RNA Second Strand Synthesis Module
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240 County Road
Ipswich, MA 019382723Tel 9789275054
Fax 9789211350www.neb.com
info@neb.comNew England Biol abs
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How to fill out nebnext ultra ii directional
How to fill out nebnext ultra ii directional
01
To fill out NebNext Ultra II Directional Kit, follow these steps:
02
Thaw the reagents and mix them gently before use.
03
Prepare the sample DNA by shearing it to the desired size.
04
Denature the DNA sample and cool it down on ice.
05
Prepare the End Repair Reaction Mix by combining the components as instructed.
06
Add the End Repair Reaction Mix to the DNA sample and mix gently.
07
Incubate the reaction mix at the specified temperature for the recommended time.
08
Purify the end-repaired DNA using the provided purification beads or column.
09
Prepare the A-Tailing Reaction Mix and add it to the purified DNA.
10
Incubate the reaction mix at the specified temperature for the recommended time.
11
Purify the A-tailed DNA using the provided purification beads or column.
12
Prepare the Ligation Reaction Mix and add it to the purified, A-tailed DNA.
13
Incubate the reaction mix at the specified temperature for the recommended time.
14
Purify the ligated DNA using the provided purification beads or column.
15
Prepare the DNA Polymerase Reaction Mix and add it to the purified, ligated DNA.
16
Incubate the reaction mix at the specified temperature for the recommended time.
17
Purify the completed library DNA using the provided purification beads or column.
18
Proceed with the sequencing or downstream applications as desired.
Who needs nebnext ultra ii directional?
01
NebNext Ultra II Directional is required by molecular biologists and researchers who need to prepare directional libraries for next-generation sequencing applications.
02
It is designed for applications such as RNA-seq, small RNA-seq, ChIP-seq, DNA sequencing, and library construction.
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What is nebnext ultra ii directional?
NebNext Ultra II Directional is a next-generation sequencing library preparation kit for directional RNA-Seq.
Who is required to file nebnext ultra ii directional?
Researchers and laboratories performing RNA sequencing experiments may be required to use the NebNext Ultra II Directional kit.
How to fill out nebnext ultra ii directional?
To fill out the NebNext Ultra II Directional kit, follow the manufacturer's protocol and guidelines for library preparation and sequencing.
What is the purpose of nebnext ultra ii directional?
The purpose of NebNext Ultra II Directional is to generate high-quality, strand-specific RNA-seq libraries for various sequencing applications.
What information must be reported on nebnext ultra ii directional?
Information such as sample name, adapter sequences, primer sequences, and other relevant sequencing data must be reported when using the NebNext Ultra II Directional kit.
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