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Infinite HD Assay for Methylation Lab Tracking Form Project: Batch: Image Date: 2Make MSA4 (PreAmp)Date/Time: BCD Plate: Operator: Robot: (1) (2) (3) Standard BCD Plate: EDNA Plate: Standard EDNA
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01
Step 1: Prepare the samples by isolating DNA and converting it to bisulfite converted DNA
02
Step 2: Quantify the bisulfite converted DNA using a fluorescent-based assay
03
Step 3: Amplify the bisulfite converted DNA using whole-genome amplification
04
Step 4: Fragment the amplified DNA into smaller pieces
05
Step 5: Purify the fragmented DNA and remove any impurities
06
Step 6: Prepare the DNA for hybridization by labeling it with a fluorescent dye
07
Step 7: Hybridize the labeled DNA to the Infinium BeadChip array
08
Step 8: Wash off unhybridized DNA and stains from the BeadChip
09
Step 9: Scan the BeadChip to detect the fluorescent signals
10
Step 10: Analyze the data using appropriate software to determine methylation levels at specific genomic loci

Who needs infinium hd methylation assay?

01
Researchers studying epigenetics and DNA methylation patterns
02
Scientists interested in understanding the role of DNA methylation in various diseases
03
Clinical laboratories performing DNA methylation analysis for diagnostic or prognostic purposes
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The Infinium HD Methylation Assay is a high-throughput technology used to profile DNA methylation across the genome, allowing researchers to understand gene regulation and epigenetic modifications.
The Infinium HD Methylation Assay is typically required for researchers or organizations performing methylation studies, particularly in the context of genetic research, cancer studies, or epigenetics.
To fill out the Infinium HD Methylation Assay, follow the provided protocols which include preparing your sample DNA, performing bisulfite conversion, and using the assay kits as per the manufacturer's instructions.
The purpose of the Infinium HD Methylation Assay is to provide comprehensive information about DNA methylation patterns, which can help in understanding various biological processes and diseases, such as cancer.
Information that must be reported includes the DNA methylation percentages at specific loci, sample identifiers, assay conditions, and any relevant metadata associated with the samples.
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