Get the free FISH for Breast HER2 Neu request form - BC Cancer Agency - bccancer bc

Addressograph HER 2 FISH TEST REQUISITION Shaded fields must be completed LEGIBLY (patient demographics may be addressographed instead). Patient Name(last, first) PhD Date of Birth’d/m/y) Sex M

How to fill out fish for breast her2

How to fill out fish for breast her2?

1. Start by collecting a tissue sample from the breast of the patient suspected to have breast her2. This can be done through a biopsy or during surgery.
2. Place the tissue sample in a fixative solution, such as formalin, to preserve the cellular structure.
3. The sample then needs to be processed in a laboratory setting, where it will be embedded in a paraffin block for sectioning.
4. Thin sections of the tissue sample are cut and placed on glass slides. These slides will be used for the fluorescence in situ hybridization (FISH) assay.
5. The next step is to deparaffinize the tissue sections using xylene or other clearing agents. This removes the paraffin wax and prepares the tissue for the hybridization process.
6. Prepare the hybridization mixture, which contains specific fluorescent probes that target the HER2 gene in the breast tissue sample.
7. Apply the hybridization mixture to the tissue sections on the slides, making sure each section is evenly covered. Incubate the slides in a hybridization oven to allow the probes to bind to the target DNA.
8. After the hybridization step, the slides are washed to remove any unbound probes.
9. Finally, the slides are examined under a fluorescent microscope to visualize the bound probes. The presence of multiple copies of the HER2 gene indicates amplification, which is characteristic of breast her2.

Who needs fish for breast her2?

1. Patients diagnosed with breast cancer may need a FISH test to determine the status of the HER2 gene in their tumor cells. This helps guide treatment decisions, as HER2-positive breast cancers are more aggressive and may require targeted therapies like trastuzumab.
2. Physicians and oncologists rely on FISH testing to accurately classify breast cancers and determine the appropriate treatment approach. The results of the FISH test provide valuable information about the HER2 gene amplification and help tailor treatment plans accordingly.
3. Researchers and scientists studying breast cancer also use FISH testing to investigate the role of the HER2 gene and its amplification in the development and progression of the disease. FISH analysis helps deepen our understanding of breast cancer biology, leading to advancements in treatment strategies.

For pdfFiller’s FAQs

What is fish for breast her2?

Fish for breast her2 is a test that detects gene abnormalities in breast cancer cells.

Who is required to file fish for breast her2?

Medical professionals or healthcare providers are responsible for performing and filing fish for breast her2.

How to fill out fish for breast her2?

Fish for breast her2 is typically filled out by conducting a lab test on a tissue sample from the breast cancer cells.

What is the purpose of fish for breast her2?

The purpose of fish for breast her2 is to determine the HER2 status of breast cancer cells, which helps in treatment planning.

What information must be reported on fish for breast her2?

The report must include the HER2 status of the breast cancer cells, indicating whether they are positive, negative, or equivocal.

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