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STANFORD UNIVERSITY Research Consent Form Protocol Director: Dr. John Protocol Title: Subject Disorders Biobanking Use Only Approval Date: February 11, 2020, Expiration Date: February 11, 2021Database

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How to fill out clonally expanded b cells

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Collect the clonally expanded B cells from the individual

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Isolate the B cells using techniques such as fluorescence-activated cell sorting (FACS)

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Culture the isolated B cells in a suitable media to promote their expansion

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Monitor the growth and expansion of the B cells over time

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Characterize the clonally expanded B cells using techniques such as flow cytometry or sequencing to confirm their clonal nature

Who needs clonally expanded b cells?

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Individuals with immune system disorders who may benefit from targeted therapy using clonally expanded B cells

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Researchers studying the immune response and developing new immunotherapies

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What is clonally expanded b cells?

Clonally expanded B cells are cells that are derived from a single ancestor cell and have multiplied to form a large population with identical receptors.

Who is required to file clonally expanded b cells?

Researchers studying immune responses or diseases related to B cells are typically required to file clonally expanded B cells.

How to fill out clonally expanded b cells?

Clonally expanded B cells can be filled out by analyzing the DNA sequences of the B cell receptors to determine clonal expansion.

What is the purpose of clonally expanded b cells?

The purpose of studying clonally expanded B cells is to understand immune responses, diseases, and the diversity of the B cell repertoire.

What information must be reported on clonally expanded b cells?

Information such as the clonal expansion size, diversity of B cell receptors, and any potential mutations must be reported on clonally expanded B cells.

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