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Mouse IL12 (P40/P70) ELISA Kit Catalog No: I.CKM052Size:1 x 96 testsIntroductionThe Cell Sciences Mouse IL12 (P40/P70) ELISA (EnzymeLinked Immunosorbent Assay) kit is an in vitro enzymelinked immunosorbent
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01
Thaw all reagents to room temperature before use.
02
Prepare all reagents, samples, and standards as instructed in the kit manual.
03
Add samples, standards, and controls to the appropriate wells on the ELISA plate.
04
Incubate the plate for the specified amount of time at the specified temperature.
05
Wash the plate thoroughly according to the kit manual instructions.
06
Add the detection antibody to each well and incubate again.
07
Wash the plate again and add the substrate solution.
08
Incubate and then stop the reaction by adding the stop solution.
09
Read the absorbance of each well at the specified wavelength and calculate the concentration of IL-12 p70 in the samples using standard curve.

Who needs il-12 p70 elisa kitmouse?

01
Researchers studying the immune response in mice
02
Laboratories conducting research on inflammatory diseases
03
Medical professionals monitoring cytokine levels in mouse models of diseases
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The IL-12 p70 ELISA Kit for mouse is a laboratory assay used to measure the levels of IL-12 p70, a cytokine involved in immune response, specifically in mouse samples.
Researchers and laboratory personnel conducting experiments involving the assessment of IL-12 p70 levels in mouse samples are required to use the IL-12 p70 ELISA Kit for mouse.
Filling out the IL-12 p70 ELISA kit involves preparing samples according to the kit instructions, adding standards and samples to the wells, incubating, washing, adding detection antibody, incubating again, and finally measuring the absorbance.
The purpose of the IL-12 p70 ELISA Kit for mouse is to quantify the concentration of IL-12 p70 cytokine in biological samples, aiding in the study of immune responses and disease mechanisms.
The information that must be reported includes sample identification, standard curve values, optical density readings, calculated concentrations of IL-12 p70 in the samples, and any relevant experimental conditions.
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