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One way of increasing the optical resolving power of the microscope is to use immersion liquids between the front lens of the objective and the cover slip. Most objectives in the magnification range between 60x and 100x (and higher) are designed for use with immersion oil.
The maximum magnification of light microscopes is usually ×1500, and their maximum resolution is 200 nm, due to the wavelength of light. An advantage of the light microscope is that it can be used to view a variety of samples, including whole living organisms or sections of larger plants and animals.
The resolution of the light microscope cannot be small than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 Am), the objects which are, at most, about 0.2 Am.
The limit of resolution (or resolving power) is a measure of the ability of the objective lens to separate in the image adjacent details that are present in the object. It is the distance between two points in the object that are just resolved in the image.
The primary factor in determining resolution is the objective numerical aperture, but resolution is also dependent upon the type of specimen, coherence of illumination, degree of aberration correction, and other factors such as contrast-enhancing methodology either in the optical system of the microscope or in the
The resolution of a specimen viewed through a microscope can be increased by changing the objective lens. The objective lenses are the lenses that protrude downward over the specimen. Grasp the nose piece. The nose piece is the platform on the microscope to which the three or four objective lenses are attached.
While bigger is often better, magnification can be meaningless if the necessary resolution is lacking as Jackson once again demonstrates. So, resolution is the ability of a system to define detail, and this becomes increasingly important the more you magnify something.
Magnification is the ability to make small objects seem larger, such as making a microscopic organism visible. Resolution is the ability to distinguish two objects from each other. Light microscopy has limits to both its resolution and its magnification.

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