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2019-03-12
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2018-12-08
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2020-04-27
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How do I get a transcript of DNA?
Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to make an RNA molecule. Transcription is performed by enzymes called RNA polymerases, which link nucleotides to form an RNA strand (using a DNA strand as a template).
How do I get my transcript from DNA to mRNA?
Transcription takes place in the nucleus. It uses DNA as a template to make an RNA molecule. RNA then leaves the nucleus and goes to a ribosome in the cytoplasm, where translation occurs. Translation reads the genetic code in mRNA and makes a protein.
How does DNA make mRNA?
DNA to RNA Transcription. The RNA to which the information is transcribed is messenger RNA (mRNA). The process associated with RNA polymerase is to unwind the DNA and build a strand of mRNA by placing on the growing mRNA molecule the base complementary to that on the template strand of the DNA.
How do you find mRNA from DNA?
In order to determine the gene sequence based off an mRNA template, you can simply do the reverse. You would match up DNA nucleotides with the complementary RNA nucleotides. You can also determine the sequence of the coding strand of DNA by simply changing the RNA U's into DNA T's.
How do you transcribe and translate a strand of DNA?
Step 1: DNA transcription. Take the strand of the provided DNA sequence and transcribe into the messenger RNA by replacing A with U, T with A, G with C and C with G. The resulting mRNA should be complimentary to the DNA. Step 2: DNA translation. tRNA reads the genetic information in mRNA in form of codon.
Which DNA strand is used for transcription?
Transcription uses one of the two exposed DNA strands as a template. This strand is called the template strand. The RNA product is complementary to the template strand and is almost identical to the other DNA strand, called the contemplate (or coding) strand.
Which strand is used for transcription?
One strand of the DNA, the template strand (or noncoding strand), is used as a template for RNA synthesis. As transcription proceeds, RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create an RNA copy (which elongates during the traversal).
Does it matter which DNA strand is transcribed?
Both the strands of a DNA can get transcribed. It depends on where the genes to form the proteins required are present. So, consider a DNA helix with one strand of 35 as A, and it's antiparallel partner as B. And there are genes on both the strands.
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