Drosophila is a relatively insensitive model for examining these interactions because it lacks both glutamatergic transmission and synaptogenesis, but this lack has not prevented efforts in recent years to dissect the interplay between stress-induced alterations in neurogenesis and depression-like behavior in Drosophila. We examined the relationship between neurodevelopmental processes in the midbrain using genetic approaches and Drosophila model systems to establish a link between neurodevelopmental processes in the midbrain and the stress response via analysis of microtubule cytoskeleton dynamics in presynaptic terminals. We measured dendrite elongation and synaptic retention rate (RET/RET) using in vivo cell-based recordings in cultured hippocampus neurons with the inducible neurogenin-5. Dendritic length and dendrite width (EDW/EDW) by the Drosophila osmotic minipump (ODM)-based microtubule-based recording were compared with those in the same cells recorded with the inducible neurotrophin-1 receptor (NT1R). We also examined the contribution of the microtubule-related protein microtubule-associated protein 3 (MAP3) and its receptor (MAP3R) to the regulation of dendritic microtubule dynamics. We identified significant positive correlations (P < .05) between dendritic length (E D) and RET/RET values (P < .05) for both MAP3R-dependent and -resistant MAP3 proteins and MAP3-resistant MAP3 proteins for both stress-sensitive and stress-resistant synaptogenic processes. E D represents the fractional area (FEW; mean ± SE; n = 6 neurons per group) surrounding dendritic spines that is larger than D, defined as the ratio of the number of dendritic spines per neuron (D) to the amount of total cells in the osmotic minipump. When analyzed as a function of MAP3R strength, MAP3R-resistant MAP3 proteins (MAP3-R), both with and without MAP3R-specific knockdown (MAP3-/-), were found to be involved in the regulation of dendritic length. We suggest that MAP3-R may mediate Drosophila neurogenesis or may be a mediator of antidepressant-Like Behavior.
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Journal of Neuroscience Methods 162 (2007) 198?205 In vivo assay of presynaptic microtubule cytoskeleton dynamics in Drosophila Yanking An, Kendal Roadie Department of Biological Sciences, Vanderbilt
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