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My name is Louie LDE, and I'm consultant hematologist at rather hospital in the UK today we are going to-do a quick morphology review of the blood film which part of the blood film should you look at to the right this ISA thin part of the blood film or red blood cells are spaced apart and therein no preservation of central pallor don't look here to the left this is thick part of a blood film where the red blood cells are overlapping each thereon×39’t look there as well in the middlethat'’s thcenterre of the blood film toucan see a good preservation of central pallor, and you'd allow some degree of overlapping of red blood cells this is the area we usually look at we will start by doing a quick review of the white blood cell morphology we will review the normal white blood cells in the blood film, and we will show you how to engage by immature white blood cell sand reactive lymphocytes white blood cells are divided into granulocytes andagranulocytesgranular sites are typically neutrophilseosinophils and basophils agranulocytesare divided into lymphocytes and 1osites neutrophil before we start talking about inter field we need to give background on staining why do red blood cells stay in red and the nucleus of all white blood cells turn blue and the answer is what--right congestion is the most widely used stain it has years in which stains basic materials red, and it has got methylene blue which stains acidic material blue, so nucleus of white blood cells will have lots of nucleic acids, and therefore it is acidic and twill stain blue now why the neutrophils are calledneutrophils because you have gods this tiny little manuals, and they have neutral standing which means they stay in between red and blue the easiest way to look at the neutral field is by looking ads in nucleus this nucleus here will have two or three loops and can be in different shapes and that×39’s why it is sometimes called polymorphonuclear cell use you know fields are easily recognizable cells they have large red granules and the nucleus is almost always obscured byte granulesbasophils are rare to have large paregoric granules lymphocyte is same size as a red blood cellist is almost nucleus with a really tiny room of site class monocle is a large cell it has large nucleus and large cytoplasm it's just three times as larges a lymphocyte or red blood cell it has this indentation you don't really have to have that we can×39’t horse show inmonocyte to call it a monocle just an indentation is enough now we will walkabout immature white blood cells this picture from bone marrow, and you can seethe big cells here to the top with high nuclear cytoplasmic ratio and visible nuclei these are blasts as the cell matures the nucleus gets smaller and the next stage in maturation ispromyelocytes you can see clearly to the left a bromine aside with a parent Golgi apparatus which we call zone of half all cells which make a lot of proteins like white blood cells you need to do a LOOF lysosomal...
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