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24.1 What form of glutamic acid would you expect to predominate in: (a) strongly acidic solution? (b) strongly basic solution? (c) at its Selectric point (pi 3.2)? (d) The Selectric point of glut

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So in the laboratory you have been exposed now to probably some of the qualitative work that we can do with amino acids, so you've taken these amino acids, and you've done these qualitative tests to them to figure out what they are seen what color they change see what happens inside the test tube and identify an unknown well there's actually a better more systematic way in order to do that and one of those is using the p-values of these amino acids so how do we calculate this P R value that's kind of independent or very special almost a fingerprint to every one of these amino acids that we will be studying well the P is very easy to describe it's very easy to define, and it's very easy to calculate so let's first start with the definition so P eye basically means Selectric point and this is the pH where there is no charge on the amino acid okay now you've got to be really careful with the wording here because it will say no charge but no matter what you do to these amino acids they will always have a charge they will either be fully positive fully negative or they will be as winner on carrying a positive and a negative both, and it all depends on what kind of acid environment that you put it in so when we say no charge it's a misnomer because we always will have a charge on these amino acids what we should say here is no overall charge on the amino acid okay so what that means is that if we take the template for an amino acid structure, and it looks like this well we're seeing a positive charge here and a negative charge there and if you add them together you get no overall charge they kind of cancel each other out right it's kind of like you made it up, and you wrote a check on the same day and your bank account is going to come out zero, so one part of the molecule has a positive one part of the molecule has a negative and the positive and the negative cancel each other out that is what we say no overall charge so the amino acids that we were dealing with we gave you a rounded bout figure of the PKA's right we said that the CE o minus group has a PKA of around 2 or the COOL group has a PKA Aaron and then the PKA of the pronated amine is about nine and a half somewhere in that vicinity, but careful wording was used during that time and I said these are averages they could actually range they could go below these values, and they can go above these values and I told you to take a look in your textbook if you're working out of the seventh edition text this is Table twenty-two point three if you're working out of another version of the text the table is still in there you might just have to look for it a little, but you're going to see all the amino acids that are listed, and you will see their exact PKA values for the carboxylic end and the amino end now some of these amino acids there are groups also have a PKA because think about it, we went through some families of amino acids, and we said that some of these were acidic in nature and some of...

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