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UDC 616-074+577.113 Development and Application of DNA Array (GD-700) for Congenital Anomaly Syndromes Yoshihide IRAQI×, Suzuki ISHIM×, Die FUJIWARA×, JunyaYESHIVAA×, Amato HIROSHI×, Took MORE×,

Therefore, the results of these studies need to be evaluated to be more precise. The CGH method offers the possibility of determining the extent of the chromosome disorders and the genomic changes at a subcellular level. The approach is applied to examine the DNA sequence and the structural organization of the chromosomal abnormality. The study of CGH has been successfully applied to study the basis of autosomal and X-chromosomal genetic disorders and to develop a genetic diagnosis for the diagnosis of congenital anomaly syndromes. The study of CGH has also been successfully applied to study human tumor development. Several reports indicate that the CGH has its application in an arrayed array to study the genetics of human chromosomal anomalies. Using the CGH method we have been able to determine the number of chromosomal regions with nonrecombination and rearrangement. The results of a CGH analysis for congenital anomaly syndromes in newborns and children are discussed. Keywords: CGH, Chromosomal rearrangements, Human chromosome abnormalities, Maternal cancer, Maternal birth defects Introduction The analysis of the structure of genes and chromosomal rearrangements are very important biological discoveries. The CGH method (Cardigan and Barrow, 1990) has become widely used in the search of genetic disorders and genetic conditions. Using the technique, the extent of nonrecombination and rearrangements of chromosomes can be determined. The method can also be applied to analyze the genomic changes in different populations, in order to ascertain the extent of the genetic alterations. The main features of CGH are the separation of DNA, which is an important step for the detection of DNA alterations. The method can distinguish between doublet rearrangements, which require the use of the complementary material and between double and triplet rearrangements, which require a DNA segment containing the cnidarian/bilirubin genes. The method was successfully applied in the analysis of several rare genetic diseases, namely CMT1 (Sachs, 1993), a syndrome of the central nervous system that can be caused by mutations in three different genes (Allure et al., 2003). CGH in newborns and children The CGH method can be applied in newborns and children of any age. It has been applied to the analysis of DNA of Down's syndrome (Hajdull-Uzun et al., 1998; Ellington et al., 2003).

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