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Adapter sequence hybridization activated with vaccinia DNA topoisomerase I for introduction of T7 promoter into PCR products.

Digoxigenin-labeled probes were synthesized onto polystyrene microcells and then digoxigenin was used to label mRNA that had been transcribed. PCR products were then digoxigenin-linked onto each digoxigenin-labeled RNA probe as a template for transcription. Twenty-six pairs of DNA probes were synthesized for each PCR condition. The number of pairs of digoxigenin-linked probes synthesized was not specified, but probably included all PCR combinations that allowed for appropriate amplification with the appropriate probe. For each PCR condition a blank control was also used. Results and Discussion Human T7 RNA DNA probes were obtained from ATC. All digoxigenin-labeled transcripts were screened for the presence of recombination elements. All digoxigenin-linked digoxigenin probes, except one was present in the digoxigenin-labeled transcript (Figure). Thus, the digoxigenin-linked digoxigenin probes that were present in the mRNA transcript can be excluded as the result of recombination. Open in a separate window The digoxigenin-labeled transcripts were used as template DNA to synthesize digoxin probe for PCR amplification using CDA and 5'-3'-3' exonuclease-labelled sense and CRNA probes (Figure). After amplification with appropriate primer pairs or digoxigenin in triplicate or in single- or paired-end oligonucleotides, a specific oligonucleotide was designed for probe synthesis of single digoxigenin-linked exons. Degeneration of T7 RNA Probes This experiment was repeated with DNA probes for human cytomegalovirus RNA (HCMV-H1) and for p53 protein, p16INK4a (p16in) DNA probe and p16INK4a mRNA DNA probe. The PCR primers, probes and PCR conditions used all of these probes, except the p16INK4a probe, and the single primer was used in triplicate or in single-end oligonucleotides or hybridization as appropriate (Figure). Thus, degeneration of the digoxigenin-linked digoxigenin probes is seen in all the RNA DNA digoxigenin probes, and the degeneration of the digoxigenin-linked digoxigenin probes is also seen in the single probe (Figure).

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DNA topoisomerase activated adapter is a type of adapter molecule designed to facilitate the activation of DNA topoisomerases, which are enzymes that regulate the topology of DNA molecules.

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What is the purpose of dna topoisomerase activated adapter?

The purpose of a DNA topoisomerase activated adapter is to facilitate the activation of DNA topoisomerases, which are enzymes that play a critical role in regulating the topology of DNA molecules. By providing a suitable adapter, the efficiency and specificity of DNA topoisomerase reactions can be enhanced.

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The specific information to be reported on a DNA topoisomerase activated adapter may vary depending on the requirements set by the relevant regulatory bodies or research institutions. Typically, the adapter may include details such as the sequence, concentration, and other relevant parameters required for the activation of DNA topoisomerases.

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