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US006114598A United States Patent 19 11 Kucherlapati et al. 54 45 GENERATION OF KETOGENIC ANTIBODIES 75 Inventors: AJU Kucherlapati, Darren, Conn.; AYA Jakobovits, Menlo Park, Calif; Sue Balfour,

How to fill out generation of xenogeneic antibodies

How to fill out generation of xenogeneic antibodies?

1. Start by selecting suitable xenogeneic sources, such as mice or rabbits, that will produce the desired antibodies.
2. Obtain the necessary ethical approvals and permits for using animals in antibody generation.
3. Immunize the chosen xenogeneic host animal with the target antigen. This can involve injecting the antigen into the animal or using alternative methods like DNA immunization.
4. Allow sufficient time for the host animal's immune system to mount a response and produce antibodies against the target antigen.
5. Collect blood or serum samples from the host animal, containing the generated xenogeneic antibodies.
6. Isolate the xenogeneic antibodies from the collected samples using techniques like protein purification or affinity chromatography.
7. Characterize the isolated antibodies for specificity, affinity, and functionality through methods like ELISA, Western blot, or functional assays.
8. Store the purified xenogeneic antibodies properly to maintain their stability and functionality.

Who needs generation of xenogeneic antibodies?

1. Researchers and scientists working in the fields of biotechnology, immunology, and pharmaceuticals often require xenogeneic antibodies for various applications.
2. Pharmaceutical companies may use xenogeneic antibodies in drug development, target validation, and diagnostics.
3. Academic institutions and research laboratories may utilize xenogeneic antibodies in studies investigating disease mechanisms, immune responses, or therapeutic interventions.
4. Clinicians and healthcare professionals may utilize xenogeneic antibodies in diagnostic tests or treatments for certain diseases or conditions.
5. Veterinary researchers may use xenogeneic antibodies to study animal diseases and develop vaccines or therapeutics for veterinary purposes.
6. Industries involved in the production of diagnostic kits, research reagents, or antibody-based products may require xenogeneic antibodies for their manufacturing processes.

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