Get the free Use of Luciferase Chimaera to Monitor PLCz Expression

Figure 2. 2a shows the relative changes in fluorescence from eggs injected with OGBD and PLC -luc cRNA. Of Oregon Green BAPTA dextran is shown. The oscillations in fluorescence indicate intracellular Ca2 oscillations are occurring. 14. Campbell K. and Swann K. 2006 Ca2 fertilization. Devel. Biol. 298 225 53. 15. Carroll J. Swann K. To image intracellular Ca2 in eggs injected with OGBD we monitor fluorescence for the period during which Ca2 signals occur 5 h. Swann K. and Lai F.A. 2002 PLC a...

How to fill out use of luciferase chimaera

How to fill out use of luciferase chimaera

1. Step 1: Start by preparing the luciferase chimaera solution according to the manufacturer's instructions.
2. Step 2: Carefully review the protocol and safety precautions before beginning the experiment.
3. Step 3: Use sterile techniques and appropriate laboratory equipment to handle the luciferase chimaera solution.
4. Step 4: Determine the appropriate concentration and volume of luciferase chimaera to use for your specific application.
5. Step 5: Prepare the sample or cell culture to be treated with the luciferase chimaera.
6. Step 6: Add the desired amount of luciferase chimaera to the sample or cell culture.
7. Step 7: Incubate the sample or cell culture with the luciferase chimaera for the recommended time and temperature.
8. Step 8: Follow the specific detection method or protocol to measure luciferase activity or expression.
9. Step 9: Record and analyze the results obtained from the luciferase chimaera experiment.
10. Step 10: Dispose of any waste materials and clean the laboratory equipment properly.

Who needs use of luciferase chimaera?

1. Researchers studying gene expression and regulation can benefit from the use of luciferase chimaera.
2. Scientists conducting drug discovery and development research often utilize luciferase chimaera for various assays.
3. Biotechnology companies and pharmaceutical companies may need to use luciferase chimaera for screening and evaluating potential drug candidates.
4. Investigators studying protein-protein interactions and signaling pathways can employ luciferase chimaera in their experiments.
5. Geneticists and molecular biologists investigating promoter activity and transcriptional regulation may require the use of luciferase chimaera.
6. Researchers in the field of synthetic biology and genetic engineering can utilize luciferase chimaera to engineer gene expression systems.
7. Scientists involved in studying cellular processes, such as apoptosis or circadian rhythms, can incorporate luciferase chimaera into their investigations.

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