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Human Reproduction, Vol.26, No.9 pp. 2461 2472, 2011 Advanced Access publication on June 24, 2011, DOI:10.1093/humrep/der196ORIGINAL ARTICLE Reproductive biology In Vito development of secondary follicles
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01
First, gather all necessary materials including a culture dish, a suitable culture medium, and embryos.
02
Next, prepare the culture dish by adding a thin layer of the culture medium.
03
Then, carefully place the embryos in the center of the dish using a sterile pipette.
04
Afterward, cover the dish and place it in an incubator set at the appropriate temperature and humidity.
05
Monitor the development of the embryos daily and record any observations.
06
Regularly add fresh culture medium to supply nutrients to the developing embryos.
07
Continue the incubation process until the desired stage of development is reached.
08
Finally, carefully transfer the embryos to a transfer dish for further experimentation or use.

Who needs in vitro development of?

01
Scientists conducting research on embryonic development.
02
Biologists studying the growth and differentiation of cells.
03
Medical professionals involved in reproductive procedures such as IVF (in vitro fertilization).

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