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Susceptibility of Mycobacterium abscesses to Antimycobacterial Drugs
in Preclinical Models
Andes ObregnHenao, a Kimberly A. Arnett, a Marcela HenaoTamayo, a Lisa Missouri, an Elizabeth Crasser, a
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How to fill out susceptibility of mycobacterium abscessus
How to fill out susceptibility of mycobacterium abscessus
01
To fill out the susceptibility of Mycobacterium abscessus, follow these steps:
02
Collect the clinical specimen, such as sputum, bronchial washings, or tissue biopsies.
03
Process the specimen according to the standard laboratory procedures.
04
Inoculate the processed specimen onto appropriate growth media, like Middlebrook 7H10 or 7H11 agar.
05
Incubate the culture at a suitable temperature (typically 30-37°C) for the recommended duration.
06
When growth is observed, subculture the isolates on fresh media to obtain pure cultures.
07
Perform the susceptibility testing using a method like the agar dilution or broth dilution method.
08
Prepare the antimicrobial agents as per the manufacturer's instructions and dilute them to the desired concentrations.
09
Apply the diluted antimicrobial agents to the culture plates or broth tubes in appropriate concentrations.
10
Incubate the culture plates or tubes for the recommended time period.
11
Evaluate the growth of Mycobacterium abscessus and record the Minimum Inhibitory Concentration (MIC) values.
12
Interpret the MIC values to determine the susceptibility or resistance of the bacteria to different antimicrobial agents.
13
Report the results accurately and communicate them to the healthcare provider.
Who needs susceptibility of mycobacterium abscessus?
01
Susceptibility testing of Mycobacterium abscessus is necessary for the following individuals:
02
- Patients with confirmed or suspected infections caused by Mycobacterium abscessus.
03
- Individuals requiring targeted antibiotic therapy for Mycobacterium abscessus infections.
04
- Healthcare providers and researchers studying the antimicrobial susceptibility patterns of Mycobacterium abscessus.
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