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User manualHoefer IEF100 Selectric Focusing UnitumIEF100IM/Rev. B0/0712Page Finder Important Information.................................................................ii Waste Electrical And Electronic

How to fill out isoelectric focusing unit

How to fill out isoelectric focusing unit

1. To fill out the isoelectric focusing unit, follow these steps:
2. Begin by preparing the gel mixture according to the manufacturer's instructions.
3. Carefully pour the gel mixture into the gel cassette, ensuring there are no air bubbles.
4. Insert the appropriate comb into the gel cassette to create the sample wells.
5. Allow the gel to solidify for the recommended amount of time.
6. Prepare the reservoir buffer and fill the anode and cathode chambers with the buffer.
7. Carefully remove the comb from the gel cassette, ensuring not to disturb the wells.
8. Place the gel cassette into the isoelectric focusing unit, ensuring proper alignment with the electrodes.
9. Connect the leads from the power supply to the appropriate electrodes.
10. Set the desired voltage and run time for the isoelectric focusing process.
11. Start the run and monitor the progress to ensure successful separation of proteins based on their isoelectric points.
12. Once the run is complete, carefully remove the gel cassette from the unit and proceed with further protein analysis or staining techniques.

Who needs isoelectric focusing unit?

1. Isoelectric focusing unit is needed by researchers and scientists in the field of molecular biology, biochemistry, and proteomics.
2. It is commonly used in protein analysis to separate proteins based on their isoelectric points.
3. This technique is particularly useful in studying protein charge heterogeneity and can help identify different isoforms or variants of proteins.
4. Researchers who are interested in studying protein expression and purification, protein structure and function, and protein modifications often rely on isoelectric focusing units.
5. It is also utilized in various research applications, such as protein fingerprinting, detection of post-translational modifications, and protein characterization.
6. Overall, anyone involved in protein research and analysis that requires separation of proteins based on isoelectric points could benefit from using an isoelectric focusing unit.

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