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Pro. Natl. Acid. Sci. USA
Vol. 76, No. 12, pp. 64256429, December 1979Cell BiologyTetrodotoxinsensitive sodium channels in normal human fibroblasts
and normal human alike cells
(sodium ionosphere/veratridine/scorpion
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To fill out tetrodotoxin-sensitive sodium channels, follow these steps:
02
Begin by obtaining a suitable cell line or tissue sample that contains tetrodotoxin-sensitive sodium channels.
03
Prepare a solution of tetrodotoxin at the desired concentration. Tetrodotoxin is a potent neurotoxin commonly found in pufferfish.
04
Add the tetrodotoxin solution to the cell line or tissue sample and incubate for a sufficient period to allow the toxin to bind to the sodium channels.
05
Rinse the sample to remove any excess tetrodotoxin and ensure that only the bound channels remain.
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The filled tetrodotoxin-sensitive sodium channels can now be used for further experimentation or study.
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