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SURGICAL PATHOLOGY HISTOLOGY STAINING MANUAL MINERALS AND PIGMENTSDate: Page: 1 of 2COPPER ROXANNE METHOD PURPOSE: To identify copper deposits, such as in Wilson's Disease, left in the liver. PRINCIPLE:
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To fill out the value of histochemical stains, follow these steps:
02
Prepare the histochemical staining solution according to the specific protocol you are using.
03
Collect the tissue or specimen that needs to be stained.
04
Place the tissue or specimen on a slide and ensure it is properly fixed.
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Add the histochemical staining solution to completely cover the tissue or specimen.
06
Incubate the slide with the staining solution according to the recommended time and temperature.
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After the incubation period, carefully rinse the slide with appropriate buffer or water to remove excess staining solution.
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Dehydrate the slide by placing it in graded alcohols and then clear in xylene or a xylene substitute.
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Mount the slide with a suitable mounting medium and cover slip.
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Observe the stained tissue or specimen under a microscope and document the results.
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Record the specific details of the histochemical staining procedure, including the staining solution used, incubation time and temperature, and any observations or findings.
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Repeat the process for additional tissue or specimens, if needed.
Who needs value of histochemical stains?
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Value of histochemical stains is needed by various professionals in the field of biology and medicine, including:
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- Histologists: They use histochemical stains to study the cellular composition and structure of tissues.
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- Pathologists: They utilize histochemical stains to analyze and diagnose diseases based on cellular changes.
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- Researchers: They rely on histochemical stains to investigate specific cellular processes and identify biomarkers.
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- Medical professionals: They may use histochemical stains to aid in the detection and characterization of tumors and other abnormal tissue changes.
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- Forensic experts: They employ histochemical stains to analyze biological samples in criminal investigations or identification cases.
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- Students: Histochemical stains are often taught and utilized in educational settings to enhance understanding of cell biology and histology.
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