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Quantizing ELISA
Mouse/Rat IL17F Immunoassay
Catalog Number M17F0For the quantitative determination of mouse and rat Interleukin 17F (IL17F) concentrations
in cell culture superstates, serum, and
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How to fill out mouserat il-17f quantikine elisa
How to fill out mouserat il-17f quantikine elisa
01
Prepare all necessary materials and reagents: Mouserat IL-17F Quantikine ELISA Kit, fresh samples or stored samples, ELISA plate, plate sealers, plate reader
02
Allow all reagents and samples to come to room temperature
03
Dilute the standards in the provided Standard Diluent according to the instructions provided in the kit
04
Pipette 100μl of standards, samples, and controls into the appropriate wells of the ELISA plate
05
Cover the plate with a plate sealer and incubate for the recommended time and temperature
06
Aspirate or decant the liquid from each well and wash the plate with provided Wash Buffer
07
Repeat the washing step several times
08
Add 200μl of the provided Conjugate to each well, cover the plate, and incubate for the recommended time and temperature
09
Aspirate or decant the liquid from each well and wash the plate again
10
Add 200μl of Substrate Solution to each well, cover the plate, and incubate for the recommended time
11
Add 50μl of Stop Solution to each well to stop the reaction
12
Measure the absorbance of each well at the appropriate wavelength using a plate reader
13
Use the absorbance values to plot a standard curve and determine the concentration of IL-17F in the samples using the provided software or equation
Who needs mouserat il-17f quantikine elisa?
01
Mouserat IL-17F Quantikine ELISA is typically used by researchers and scientists studying the role of IL-17F in mouse and rat models.
02
It can be used to measure the concentration of IL-17F in various biological samples, such as serum, plasma, and cell culture supernatant.
03
This ELISA kit provides a reliable and quantitative method for detecting and analyzing IL-17F, which can help in understanding its function and potential implications in various diseases and conditions.
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